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pubmed-article:8872348pubmed:abstractTextThe neural ecto-diadenosine polyphosphate hydrolase (ecto-ApnAase) from plasma membranes of Torpedo synaptic terminals is inhibited by suramin. This study was carried out by discontinuous h.p.l.c. and continuous fluorometric methods. The concentration-dependence studies showed a non-competitive mechanism for suramin in the Dixon plot, with a Ki value of 1.79 +/- 0.03 microM with respect to epsilon-(Ap3A) as the substrate and 1.69 +/- 0.05 microM and 1.86 +/- 0.06 microM for epsilon-(Ap4A) and epsilon-(Ap5A) respectively. These results indicate that suramin could be a base compound inhibiting ecto-ApnAase and providing an alternative way of studying the pharmacology of diadenosine polyphosphate receptors.lld:pubmed
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pubmed-article:8872348pubmed:articleTitleSuramin--a powerful inhibitor of neural ecto-diadenosine polyphosphate hydrolase.lld:pubmed
pubmed-article:8872348pubmed:affiliationDepartamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.lld:pubmed
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pubmed-article:8872348pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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