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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1997-3-25
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pubmed:abstractText |
The neural ecto-diadenosine polyphosphate hydrolase (ecto-ApnAase) from plasma membranes of Torpedo synaptic terminals is inhibited by suramin. This study was carried out by discontinuous h.p.l.c. and continuous fluorometric methods. The concentration-dependence studies showed a non-competitive mechanism for suramin in the Dixon plot, with a Ki value of 1.79 +/- 0.03 microM with respect to epsilon-(Ap3A) as the substrate and 1.69 +/- 0.05 microM and 1.86 +/- 0.06 microM for epsilon-(Ap4A) and epsilon-(Ap5A) respectively. These results indicate that suramin could be a base compound inhibiting ecto-ApnAase and providing an alternative way of studying the pharmacology of diadenosine polyphosphate receptors.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0007-1188
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
119
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-2
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8872348-Acid Anhydride Hydrolases,
pubmed-meshheading:8872348-Animals,
pubmed-meshheading:8872348-Cell Membrane,
pubmed-meshheading:8872348-Chromatography, High Pressure Liquid,
pubmed-meshheading:8872348-Dose-Response Relationship, Drug,
pubmed-meshheading:8872348-Electric Organ,
pubmed-meshheading:8872348-Enzyme Inhibitors,
pubmed-meshheading:8872348-Fluorometry,
pubmed-meshheading:8872348-Kinetics,
pubmed-meshheading:8872348-Suramin,
pubmed-meshheading:8872348-Synapses,
pubmed-meshheading:8872348-Torpedo
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pubmed:year |
1996
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pubmed:articleTitle |
Suramin--a powerful inhibitor of neural ecto-diadenosine polyphosphate hydrolase.
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pubmed:affiliation |
Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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