Linked Life Data

8871654

Source:http://linkedlifedata.com/resource/pubmed/id/8871654

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1996-12-17
pubmed:abstractText
Following ocular infection, herpes simplex virus type 1 (HSV-1) establishes latency in trigeminal ganglion (TG) neurons. Using reverse transcription-PCR, cytokine gene expression was analyzed in the TGs of mice infected with HSV-1. IL-2, TNF-alpha, IFN-gamma, IL-10, and RANTES mRNAs were readily detected in TGs taken from mice 7 days postinoculation (PI). Likewise, IL-2, IL-6, IL-10, and IFN-gamma protein were detected by ELISA of TG homogenates. Between 5 and 45 days PI, IL-10, IFN-gamma, TNF-alpha, and RANTES mRNAs were detected in nearly 100% of latently infected TGs (latent infection was confirmed by reverse transcription-PCR detection of HSV-1 latency-associated transcripts). T cell-associated cytokine and chemokine mRNAs (IL-2, IL-10, IFN-gamma, and RANTES) were still detected in the majority of latently infected TG samples taken between 60 and 135 days PI. In contrast, these cytokine mRNA species were rarely detected in uninfected TGs. Measurement of serum Abs to HSV-1 at different times revealed that anti-HSV-1 Ab concentrations approached a plateau in mice by 30 days PI but remained at high levels 67 and 125 days PI. Although there was molecular evidence of an ongoing immune response to HSV-1 in latently infected TG, histologic analysis indicated that very few mononuclear cells remained in the ganglion 60 days PI. Collectively, the results suggest that residual lymphocytes encounter viral Ag during HSV-1 latency with sufficient frequency to remain activated. The paradox of a persistent immune response against a latent infection is discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
157
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3542-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8871654-Animals, pubmed-meshheading:8871654-Antibodies, Viral, pubmed-meshheading:8871654-Base Sequence, pubmed-meshheading:8871654-Cell Line, pubmed-meshheading:8871654-Cercopithecus aethiops, pubmed-meshheading:8871654-Chemokine CCL5, pubmed-meshheading:8871654-Cytokines, pubmed-meshheading:8871654-DNA Primers, pubmed-meshheading:8871654-Female, pubmed-meshheading:8871654-Gene Expression, pubmed-meshheading:8871654-Herpesvirus 1, Human, pubmed-meshheading:8871654-Interferon-gamma, pubmed-meshheading:8871654-Interleukin-10, pubmed-meshheading:8871654-Interleukin-2, pubmed-meshheading:8871654-Keratitis, Herpetic, pubmed-meshheading:8871654-Mice, pubmed-meshheading:8871654-Mice, Inbred ICR, pubmed-meshheading:8871654-RNA, Messenger, pubmed-meshheading:8871654-Time Factors, pubmed-meshheading:8871654-Trigeminal Ganglion, pubmed-meshheading:8871654-Tumor Necrosis Factor-alpha, pubmed-meshheading:8871654-Vero Cells
pubmed:year
1996
pubmed:articleTitle
Persistent cytokine expression in trigeminal ganglion latently infected with herpes simplex virus type 1.
pubmed:affiliation
Department of Microbiology, Louisiana State University Medical Center, New Orleans 70112, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.