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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1996-12-5
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pubmed:databankReference |
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pubmed:abstractText |
TIA-1 and TIAR are RNA binding proteins of the RNA recognition motif (RRM)/ribonucleoprotein (RNP) family that have been implicated as effectors of apoptotic cell death. We report the structures of murine TIA-1 and TIAR (mTIA-1 and mTIAR) deduced from cDNA cloning, the mRNA and protein tissue distribution of mTIA-1 and mTIAR, and the exon-intron structures of the mTIA-1 and mTIAR genes. Both mTIA-1 and mTIAR are comprised of three approximately 100 amino acid N-terminal RRM domains and a approximately 90 amino acid C-terminal auxiliary domain. This subfamily of RRM proteins is evolutionarily well conserved; mTIA-1 and mTIAR are 80% similar to each other, and 96 and 99% similar to hTIA-1 and hTIAR, respectively. The overall exon-intron structures of the mTIA-1 and mTIAR genes are also similar to each other, as well as to the human TIA-1 gene structure. While Northern blot analysis reveals that mTIA-1 and mTIAR mRNAs have a broad tissue distribution, mTIA-1 and mTIAR proteins are predominantly expressed in brain, testis and spleen. At least two isoforms of both mTIA-1 and mTIAR are generated by alternative splicing. Murine TIA-1 isoforms including or lacking the exon 5 encoded sequences are expressed at a ratio of approximately 1:1, whereas mTIAR isoforms including or lacking the 5'-end of exon 3 sequences are expressed in a approximately 1:6 ratio. Molecular characterization of murine TIA-1 and TIAR RNA binding proteins provides the basis for a genetic analysis of the functional roles of these proteins during mammalian development.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-1326761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-1716386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-1848010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-1934064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-2505080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-2827008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-2971730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-3491324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-7523244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-7533298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-7543225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-7544399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-7568171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-7670487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-7673195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-7684991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-7906398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-7926753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-7984237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-8036511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-8146191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-8176212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-8269511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-8331337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-8396236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-8500177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-8576255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8871565-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Poly(A)-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TIA1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/TIAL1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tia1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tial1 protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3829-35
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:8871565-Amino Acid Sequence,
pubmed-meshheading:8871565-Animals,
pubmed-meshheading:8871565-Cloning, Molecular,
pubmed-meshheading:8871565-DNA, Complementary,
pubmed-meshheading:8871565-Exons,
pubmed-meshheading:8871565-Genome,
pubmed-meshheading:8871565-Humans,
pubmed-meshheading:8871565-Introns,
pubmed-meshheading:8871565-Membrane Proteins,
pubmed-meshheading:8871565-Mice,
pubmed-meshheading:8871565-Molecular Sequence Data,
pubmed-meshheading:8871565-Poly(A)-Binding Proteins,
pubmed-meshheading:8871565-Protein Conformation,
pubmed-meshheading:8871565-Proteins,
pubmed-meshheading:8871565-RNA-Binding Proteins,
pubmed-meshheading:8871565-Sequence Homology, Amino Acid
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pubmed:year |
1996
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pubmed:articleTitle |
Structure, tissue distribution and genomic organization of the murine RRM-type RNA binding proteins TIA-1 and TIAR.
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pubmed:affiliation |
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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