Linked Life Data

8869937

Source:http://linkedlifedata.com/resource/pubmed/id/8869937

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-1-29
pubmed:abstractText
Plasma obtained from lethally irradiated animals contains a megakaryocyte (MK) growth factor which has recently been identified as the ligand for the c-mpl receptor and has been named thrombopoietin (TPO). We demonstrate that post-irradiation aplastic canine plasma (PICS-J) and plasma from a human subject (ML) who was accidentally exposed to lethal irradiation, contain high levels of this activity, which support both MK proliferation and maturation in a dose-dependent manner. These plasma were far more active in stimulating human MK colony formation than other types of thrombocytopenic plasma or a number of exogenously added human recombinant cytokines and their combinations. The addition of stem cell factor (SCF), which alone has a minimal stimulatory affect, to post lethal-irradiation plasma provided a synergistic stimulation of megakaryocytopoiesis both in colony assays and liquid cultures. In colony assays, the combination of SCF with PICS-J or ML almost doubled the number of burst forming units (BFU-MK) and provided a 1.5-fold increase in colony forming units (CFU-MK). A 1.6-fold increase in the number of CD34+ BM cell-derived MK colonies was also elicited. In liquid cultures, the presence of both SCF and PICS-J or ML induced the appearance of a high proportion of CD34+ (6.56% vs 0.6% control) and CD41+ (3.5% vs 1.2% control) cells after 3 days in culture. By day 10, 66.8 x 10(4) CD41+ cells and 29.8 x 10(4) CD34+ cells were derived from 2 x 10(6) BMMC originally seeded. We propose that these unique plasma, which do not contain elevated level of IL-6, IL-3, GM-CSF, IL-1 beta, erythropoietin or SCF, probably contain high levels of TPO. The addition of SCF to the post-irradiation plasma provides a synergistic stimulation of megakaryocytopoiesis which may become relevant for future clinical application.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1357-0560
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
31-42
pubmed:dateRevised
2011-10-26
pubmed:meshHeading
pubmed-meshheading:8869937-Anemia, Aplastic, pubmed-meshheading:8869937-Animals, pubmed-meshheading:8869937-Blood Proteins, pubmed-meshheading:8869937-Bone Marrow Transplantation, pubmed-meshheading:8869937-Cell Division, pubmed-meshheading:8869937-Cells, Cultured, pubmed-meshheading:8869937-Colony-Stimulating Factors, pubmed-meshheading:8869937-Dogs, pubmed-meshheading:8869937-Drug Synergism, pubmed-meshheading:8869937-GPI-Linked Proteins, pubmed-meshheading:8869937-Hematopoiesis, pubmed-meshheading:8869937-Humans, pubmed-meshheading:8869937-Male, pubmed-meshheading:8869937-Megakaryocytes, pubmed-meshheading:8869937-Membrane Glycoproteins, pubmed-meshheading:8869937-Proteins, pubmed-meshheading:8869937-Radiation Injuries, Experimental, pubmed-meshheading:8869937-Radioactive Hazard Release, pubmed-meshheading:8869937-Stem Cell Factor, pubmed-meshheading:8869937-Whole-Body Irradiation
pubmed:year
1996
pubmed:articleTitle
Stem cell factor (SCF) synergizes with megakaryocyte colony stimulating activity in post-irradiated aplastic plasma in stimulating human megakaryocytopoiesis.
pubmed:affiliation
Department of Hematology, Hadassah University Hospital, Jerusalem, Israel.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Case Reports, Research Support, Non-U.S. Gov't