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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1996-12-2
|
| pubmed:abstractText |
Glucose plays an important role in fetal development and energy metabolism. Facilitative glucose transporter-1 (GLUT1) has been found in placenta. However, little is known about GLUT1 modulation in placental cells. To examine changes in mouse placental GLUT1 levels caused by 8-bromo-cAMP, we performed 2-deoxyglucose uptake experiments, Northern blot analysis and immunoblot analysis using a primary mouse placental cell culture. Immunohistochemical analysis showed that GLUT1 was localized to the ectoplacental cone and the labyrinth zone of mouse placentas on days 7 and 11 of pregnancy respectively. Treatment of mouse placental cells with 250 mumol/l 8-bromo-cAMP resulted in a significant (P < 0.01) decrease in glucose uptake on days 2-5 of culture. The inhibitory effect of 8-bromo-cAMP on glucose uptake was concentration-dependent. Glucose uptake was also inhibited by 100 micrograms/l cholera toxin and by 0.1 mmol/l forskolin. Northern blot and immunoblot analysis revealed that both GLUT1 mRNA and protein levels were also decreased by 8-bromo-cAMP. These findings suggest that 8-bromo-cAMP inhibits glucose transport activity in mouse placental cells in culture.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/8-Bromo Cyclic Adenosine...,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyglucose,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 1,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Slc2a1 protein, mouse
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0022-0795
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
150
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
319-27
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8869598-8-Bromo Cyclic Adenosine Monophosphate,
pubmed-meshheading:8869598-Animals,
pubmed-meshheading:8869598-Biological Transport,
pubmed-meshheading:8869598-Blotting, Northern,
pubmed-meshheading:8869598-Cells, Cultured,
pubmed-meshheading:8869598-Deoxyglucose,
pubmed-meshheading:8869598-Depression, Chemical,
pubmed-meshheading:8869598-Female,
pubmed-meshheading:8869598-Glucose,
pubmed-meshheading:8869598-Glucose Transporter Type 1,
pubmed-meshheading:8869598-Immunoblotting,
pubmed-meshheading:8869598-Immunohistochemistry,
pubmed-meshheading:8869598-In Situ Hybridization,
pubmed-meshheading:8869598-Mice,
pubmed-meshheading:8869598-Mice, Inbred ICR,
pubmed-meshheading:8869598-Monosaccharide Transport Proteins,
pubmed-meshheading:8869598-Placenta,
pubmed-meshheading:8869598-Pregnancy
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
8-Bromo-cAMP inhibits glucose transport activity in mouse placental cells in culture.
|
| pubmed:affiliation |
Department of Obstetrics and Gynecology, Osaka University Medical School, Suita, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|