Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1996-12-9
|
| pubmed:abstractText |
The human T-lymphoid cell line, CEM, was transfected with gp 160 cDNA of human immunodeficiency virus type 1 (HIV-1)pm213. Three clones expressing the envelope glycoproteins (env), designated CEM-213env1, -env4, and -env7, were isolated. These clones expressed high levels of surface gp41 and gp120, as demonstrated by flow cytometry with anti-HIV env monoclonal antibodies. Processing and function of env was shown by induction of syncytia with CD4-expressing HeLa cells and by immunoblot analysis. The env expression resulted in specific down-regulation of surface CD4 levels, supporting the role of HIV env in CD4 modulation. Furthermore, serum samples from nine of nine HIV-1-infected individuals bound specifically to the env-expressing transfectants, substantiating the presence of conserved antigenic determinants. These sera also mediated antibody-dependent cellular cytotoxicity (ADCC) of the env-expressing cell lines. The env-expressing cell lines provide a relevant, safe, and practical model for qualitative and quantitative analysis of humoral and cellular immune responses and their role in HIV-1 pathogenesis and therapy.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Envelope Protein gp120,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Envelope Protein gp160,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Envelope Protein gp41
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
1077-9450
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
13
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
117-26
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8862276-Antigens, CD4,
pubmed-meshheading:8862276-Blotting, Western,
pubmed-meshheading:8862276-Cells, Cultured,
pubmed-meshheading:8862276-Clone Cells,
pubmed-meshheading:8862276-Cytotoxicity, Immunologic,
pubmed-meshheading:8862276-Down-Regulation,
pubmed-meshheading:8862276-Epitopes,
pubmed-meshheading:8862276-Flow Cytometry,
pubmed-meshheading:8862276-Gene Expression Regulation, Viral,
pubmed-meshheading:8862276-Giant Cells,
pubmed-meshheading:8862276-HIV Envelope Protein gp120,
pubmed-meshheading:8862276-HIV Envelope Protein gp160,
pubmed-meshheading:8862276-HIV Envelope Protein gp41,
pubmed-meshheading:8862276-HIV Infections,
pubmed-meshheading:8862276-HIV-1,
pubmed-meshheading:8862276-HeLa Cells,
pubmed-meshheading:8862276-Humans,
pubmed-meshheading:8862276-T-Lymphocytes,
pubmed-meshheading:8862276-Transfection
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Development of T-cell lines expressing functional HIV-1 envelope glycoproteins for evaluation of immune responses in HIV-infected individuals.
|
| pubmed:affiliation |
Medarex, Inc., Annandale, New Jersey 08801, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|