Linked Life Data

8858601

Source:http://linkedlifedata.com/resource/pubmed/id/8858601

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-1-2
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L10666, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L17074, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U38494, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U38495, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U38496, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U38497, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U38498, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U38499, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U38500, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U38501, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U38502, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U38503, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U38505
pubmed:abstractText
Fertilization in mammals initiates "egg activation," a series of events leading to embryo development. The signal transduction events that occur as a result of sperm-egg interactions and that initiate egg activation may be analogous to a ligand-receptor-effector pathway, but the details of this signaling pathway are poorly understood. Several lines of evidence support a role for guanine nucleotide-binding regulatory proteins (G proteins) in mammalian egg activation. Prior to initiating studies to examine further the role of specific G proteins in sperm-induced mouse egg activation, we needed to define the complement of G proteins expressed in the egg. Using a reverse transcription-polymerase chain reaction (RT-PCR) assay, the relative levels of mRNAs encoding specific G protein alpha, beta, and gamma subunits were determined in meiotically incompetent oocytes, fully-grown competent oocytes, metaphase II-arrested eggs, one-, two-, and eight-cell embryos, and blastocysts. mRNA transcripts representing all of the heterotrimeric G protein families were present at all of the stages examined, and all underwent significant changes in their patterns of expression. The following heterotrimeric G protein mRNA transcripts were present in oocytes, eggs, or preimplantation embryos: G alpha q family (q, 11, and 14), G alpha 12 family (12 and 13), G alpha i family (i1, i2, i3, t2, z, and s), beta subunits 1, 2, 4, and 5, and gamma subunits 2, 3, 5, and 7. A recently described large molecular weight G protein, G alpha h (Nakaoka et al., 1994: Science 264:1593-1596), was also present, G alpha 15, G alpha t1, G alpha olf, G alpha oA, G beta 3, G gamma 1, and G gamma 8 mRNA transcripts were not detected using this method. The most common pattern of expression observed was a maturation-associated decrease followed by an increase after the two-cell stage. Some transcripts, however, were expressed at low levels until the eight-cell to blastocyst stages, whereas others were expressed at high levels in the oocyte but following maturation declined and remained at a low level throughout preimplantation development.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1040-452X
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
315-23
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
G protein gene expression during mouse oocyte growth and maturation, and preimplantation embryo development.
pubmed:affiliation
Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia 19104-6080, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.