pubmed-article:8858536 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:8858536 | lifeskim:mentions | umls-concept:C0009325 | lld:lifeskim |
pubmed-article:8858536 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
pubmed-article:8858536 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
pubmed-article:8858536 | lifeskim:mentions | umls-concept:C0699900 | lld:lifeskim |
pubmed-article:8858536 | lifeskim:mentions | umls-concept:C0243125 | lld:lifeskim |
pubmed-article:8858536 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
pubmed-article:8858536 | lifeskim:mentions | umls-concept:C0066400 | lld:lifeskim |
pubmed-article:8858536 | pubmed:issue | 5 | lld:pubmed |
pubmed-article:8858536 | pubmed:dateCreated | 1996-12-19 | lld:pubmed |
pubmed-article:8858536 | pubmed:abstractText | Measurements of the conversion of [14C]-proline to [14C]-hydroxyproline were employed to assess the effect of methyl mercaptan on intra- and extracellular metabolism of collagenous proteins in human gingival fibroblast cultures. Following a 30-min pulse, 10 ng of methyl mercaptan per ml of 95% air/5% CO2 head-space suppressed collagen synthesis by 39% and increased the intracellular degradation of newly synthesized collagen from 26% to 42%. Parallel cultures assayed for proline transport demonstrated a 29% inhibition of [14C]-proline uptake. A similar analysis of cultures exposed to methyl mercaptan for 12 h revealed an increase in intracellular degradation (20% control vs. 30% test) and a marked increase in extracellular collagenolysis (4% control vs. 55% test). While pulsing, collagen synthesis was decreased by 39%. Slab gel electrophoresis also demonstrated that treatment with methyl mercaptan caused reductions both in mature alpha 1 and alpha 2 chains of type I collagen and in type III procollagen. Identities of the procollagen species were confirmed by pepsin digestion. Reverse transcribed polymerase chain reaction was utilized to compare expression of alpha 1 chains of type I procollagen with type III procollagen and indicated suppression of mRNA synthesis for type III procollagen in cultures exposed to methyl mercaptan. | lld:pubmed |
pubmed-article:8858536 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8858536 | pubmed:language | eng | lld:pubmed |
pubmed-article:8858536 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8858536 | pubmed:citationSubset | D | lld:pubmed |
pubmed-article:8858536 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8858536 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8858536 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8858536 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8858536 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8858536 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8858536 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8858536 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8858536 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:8858536 | pubmed:month | Jul | lld:pubmed |
pubmed-article:8858536 | pubmed:issn | 0022-3484 | lld:pubmed |
pubmed-article:8858536 | pubmed:author | pubmed-author:JohnsonPP | lld:pubmed |
pubmed-article:8858536 | pubmed:author | pubmed-author:TonzetichJJ | lld:pubmed |
pubmed-article:8858536 | pubmed:author | pubmed-author:YaegakiKK | lld:pubmed |
pubmed-article:8858536 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:8858536 | pubmed:volume | 31 | lld:pubmed |
pubmed-article:8858536 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:8858536 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:8858536 | pubmed:pagination | 323-9 | lld:pubmed |
pubmed-article:8858536 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
pubmed-article:8858536 | pubmed:meshHeading | pubmed-meshheading:8858536-... | lld:pubmed |
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pubmed-article:8858536 | pubmed:meshHeading | pubmed-meshheading:8858536-... | lld:pubmed |
pubmed-article:8858536 | pubmed:year | 1996 | lld:pubmed |
pubmed-article:8858536 | pubmed:articleTitle | Effect of methyl mercaptan on synthesis and degradation of collagen. | lld:pubmed |
pubmed-article:8858536 | pubmed:affiliation | Department of Stomatology, University of California, San Francisco, USA. | lld:pubmed |
pubmed-article:8858536 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:8858536 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
pubmed-article:8858536 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8858536 | lld:pubmed |