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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
1996-11-25
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pubmed:abstractText |
The class I major histocompatibility complex (MHC) glycoprotein HLA-B27 binds short peptides containing arginine at peptide position 2 (P2). The HLA-B27/peptide complex is recognized by T cells both as part of the development of the repertoire of T cells in the cellular immune system and during activation of cytotoxic T cells. Based on the three-dimensional structure of HLA-B27, we have synthesized a ligand with an aziridine-containing side chain designed to mimic arginine and to bind covalently in the arginine-specific P2 pocket of HLA-B27. Using tryptic digestion followed by mass spectrometry and amino acid sequencing, the aziridine-containing ligand is shown to alkylate specifically cysteine 67 of HLA-B27. Neither free cysteine in solution nor an exposed cysteine on a class II MHC molecule can be alkylated, showing that specific recognition between the anchor side-chain pocket of an MHC class I protein and the designed ligand (propinquity) is necessary to induce the selective covalent reaction with the MHC class I molecule.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-1335362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-1360705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-1371238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-1525820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-1546328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-1553383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-1565634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-1718307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-1722335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-1922337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-1922338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-2257626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-4123836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-4688372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-7688130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-7836906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-8023162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-8025120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-8145819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-8177320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8855288-8476568
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10945-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8855288-Affinity Labels,
pubmed-meshheading:8855288-Arginine,
pubmed-meshheading:8855288-Aziridines,
pubmed-meshheading:8855288-Binding Sites,
pubmed-meshheading:8855288-Circular Dichroism,
pubmed-meshheading:8855288-HLA-B27 Antigen,
pubmed-meshheading:8855288-HLA-DR1 Antigen,
pubmed-meshheading:8855288-Humans,
pubmed-meshheading:8855288-Ligands,
pubmed-meshheading:8855288-Peptides,
pubmed-meshheading:8855288-Protein Binding,
pubmed-meshheading:8855288-Thermodynamics
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pubmed:year |
1996
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pubmed:articleTitle |
Covalent HLA-B27/peptide complex induced by specific recognition of an aziridine mimic of arginine.
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pubmed:affiliation |
Department of Chemistry, Harvard University, Cambridge, MA 02138, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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