Linked Life Data

8853438

Source:http://linkedlifedata.com/resource/pubmed/id/8853438

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3 Pt 2
pubmed:dateCreated
1996-12-5
pubmed:abstractText
Renal glomerular capillary tufts have been believed to arise from angiogenic ingrowth of extrinsic vessels. We found, however, that when embryonic day 12 (E12) mouse kidneys were maintained in culture for 6 days and then grafted into anterior eye chambers of adult transgenic ROSA26 host mice (which carry the beta-galactosidase transgene), glomerular endothelial cells within the grafts were predominantly of intrinsic, kidney origin. To identify potential endothelial precursors, we immunolabled kidneys with antibodies against the vascular endothelial growth factor receptor, flk-1. Numerous discrete cells expressing flk-1 were scattered throughout the nephrogenic mesenchyme of both E12 and newborn kidneys, and with development these cells became concentrated in microvessels, glomerular vascular clefts, and glomerular tufts. In adults, flk-1 was weakly expressed in glomeruli but absent elsewhere. To examine abilities of flk-1-positive cells to establish glomeruli, E12 kidneys were grafted into kidney cortices of adult and newborn ROSA26 hosts. Grafts into adults resulted in few glomeruli containing host-derived endothelium, whereas a majority of glomeruli grafted into newborns contained host cells. Cells of graft origin were found in vessels forming in renal cortices of newborn hosts, but not in adults. Our findings indicate that embryonic kidney cells expressing flk-1 are angioblasts that create microvessels and glomeruli by vasculogenesis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
271
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
F744-53
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:8853438-Animals, pubmed-meshheading:8853438-Anterior Chamber, pubmed-meshheading:8853438-Embryo, Mammalian, pubmed-meshheading:8853438-Fetal Tissue Transplantation, pubmed-meshheading:8853438-Fluorescent Antibody Technique, pubmed-meshheading:8853438-Kidney, pubmed-meshheading:8853438-Kidney Cortex, pubmed-meshheading:8853438-Lectins, pubmed-meshheading:8853438-Mice, pubmed-meshheading:8853438-Mice, Inbred C57BL, pubmed-meshheading:8853438-Mice, Transgenic, pubmed-meshheading:8853438-Neovascularization, Physiologic, pubmed-meshheading:8853438-Organ Culture Techniques, pubmed-meshheading:8853438-Plant Lectins, pubmed-meshheading:8853438-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:8853438-Receptors, Growth Factor, pubmed-meshheading:8853438-Receptors, Vascular Endothelial Growth Factor
pubmed:year
1996
pubmed:articleTitle
Evidence that embryonic kidney cells expressing flk-1 are intrinsic, vasculogenic angioblasts.
pubmed:affiliation
Department of Comparative Medicine, University of Alabama at Birmingham 35294-0019, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't