Linked Life Data

8852716

Source:http://linkedlifedata.com/resource/pubmed/id/8852716

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-12-5
pubmed:abstractText
A high-performance liquid chromatographic (HPLC) method is described for the determination of ractopamine (LY031537) in monkey plasma and swine serum. Plasma or serum (0.5 ml) was diluted with phosphate buffer pH 7.0. Ractopamine was isolated from the plasma matrix using ion exchange on a polymeric carboxylic acid solid-phase extraction cartridge followed by partitioning with ethyl acetate. An isocratic HPLC method using electrochemical detection at +700 mV was used to separate and measure ractopamine in the purified extract in 6.5 min of run time. Standard area response was linear with respect to concentration of ractopamine over the range of 0.5 to 40 ng/ml. Validation data were collected using rhesus monkey plasma and swine serum. The method precision and accuracy were evaluated in the range 1.0 to 20 ng/ml using fortified samples of monkey plasma. The method limit of quantitation was estimated at 2 ng/ml as determined in monkey plasma.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1572-6495
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
675
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
279-85
pubmed:dateRevised
2007-10-16
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Determination of ractopamine in monkey plasma and swine serum by high-performance liquid chromatography with electrochemical detection.
pubmed:affiliation
Lilly Research Laboratories, Eli Lilly and Company, Greenfield, IN 46140-0708, USA.
pubmed:publicationType
Journal Article