Linked Life Data

8851871

Source:http://linkedlifedata.com/resource/pubmed/id/8851871

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-12-10
pubmed:abstractText
The DNA segment of the Streptomyces diastaticus strain No.7 M1033 D-xylose Isomerase gene, from -192 to +581 bp according to the translation start site (tss), was cloned in Streptomyces promoter-probe plasmid pIJ4083 from recombination plasmid pUB1 and its M13 subclone S1 respectively. The protoplasts of S. lividans TK24 were transformed with the recombinant, and then detected the promoter activity by measuring the catethol dioxygenase expression. Results of S1 mapping of total RNA of S. di. M1033 indicatedthat: the transcription initiation site of D-XI gene was 40bp upstream the coding region; there was another gene encoded on the antisense strand upstream D-XI gene, with a 114-nucleotide sequence separating their coding regions. According to its DNA sequence, we believed it was the xylulose kinase gene. Data from quantitive S1 mapping also suggested that transcription of the two genes was induced by D-xylose and repressed by glucose.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0379-4172
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
239-44
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
[Promoter activity detection and transcription initiation site determination of the D-xylose isomerase gene of Streptomyces diastaticus strain No.7 M1033].
pubmed:affiliation
Department of Biology, University of Science and Technology of China Hefei.
pubmed:publicationType
Journal Article, English Abstract