Linked Life Data

8850609

Source:http://linkedlifedata.com/resource/pubmed/id/8850609

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1996-12-11
pubmed:abstractText
Intact fumonisins contain two tricarballylic acid groups and can therefore acquire a net negative charge. The anionic nature of the fumonisins is the basis behind the widely used method for cleanup of corn with strong anion exchange (SAX) columns. This property also enables the fumonisins to be separated by electrophoretic techniques which, until now, have not been applied to the analysis of fumonisins in corn. Fumonisin B1, extracted from corn with 80/20 (v/v) methanol/water and isolated with a commercially available affinity column, was derivatized with fluorescein isothiocyanate for analysis by capillary zone electrophoresis with laser-induced fluorescence detection (CZE-LIF). Recoveries from corn fortified with 0.25 to 5.0 ppm FB1 averaged 89% (range 71 to 102%). As little as 0.05 ppm FB1 could be detected in corn. For corn naturally contaminated with FB1, the CZE-LIF method compared favorably to established SAX/HPLC and C18/HPLC methods. Capillary electrophoresis can be used for quantitation of FB1 in corn, with minimal use of organic solvents and provides an additional tool for confirming fumonisin contamination.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0065-2598
pubmed:author
pubmed:issnType
Print
pubmed:volume
392
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
105-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Analysis of fumonisin B1 in corn by capillary electrophoresis.
pubmed:affiliation
Mycotoxin Research Unit, National Center for Agricultural Utilization Research USDA/ARS, Peoria, IL 61604, USA.
pubmed:publicationType
Journal Article, Comparative Study