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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1996-10-23
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pubmed:abstractText |
1. Voltage-dependent calcium currents were measured at room temperature using whole-cell patch clamp in acutely isolated somata and axon terminals of the magnocellular neurosecretory cells (MNCs) from the rat supraoptic nucleus. 2. Administration of omega-agatoxin IVA (omega-Aga IVA) blocked a high-threshold non-inactivating current. This current has an IC50 for omega-Aga IVA of 3 nM; no other types of currents were blocked at doses of up to 500 nM. 3. In the axon terminals omega-Aga IVA blocked a high-threshold current that inactivates markedly (tau approximately 448 ms), and has a much lower sensitivity to the toxin, with an IC50 of 270 nM. Unlike the somatic current, the effect of omega-Aga IVA in the terminals is largely prevented by omega-conotoxin GVIA (omega-CgTX). 4. These data suggest that MNC somata express a single type of omega-Aga IVA-sensitive calcium current similar to the P-type calcium current described in other cells. However, the omega-Aga IVA-sensitive current in axon terminals differs from both the P-type and the recently identified Q-type current in that it is also sensitive to omega-CgTX. The distinct biophysical properties of the currents in somata and axon terminals may have important physiological implications.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-1321648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-1323666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-1659003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-1660249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-1849233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-2295621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-2432227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-2537980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-2560641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-2850339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-3656152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-3774226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-6348947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-7441553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-7473220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-7524096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-7722641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-7832825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-7901765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8847634-8032915
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
489 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
383-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8847634-Animals,
pubmed-meshheading:8847634-Axons,
pubmed-meshheading:8847634-Calcium Channel Blockers,
pubmed-meshheading:8847634-Calcium Channels,
pubmed-meshheading:8847634-Dose-Response Relationship, Drug,
pubmed-meshheading:8847634-Male,
pubmed-meshheading:8847634-Patch-Clamp Techniques,
pubmed-meshheading:8847634-Rats,
pubmed-meshheading:8847634-Spider Venoms,
pubmed-meshheading:8847634-Supraoptic Nucleus,
pubmed-meshheading:8847634-Time Factors,
pubmed-meshheading:8847634-omega-Agatoxin IVA
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pubmed:year |
1995
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pubmed:articleTitle |
Distinct omega-agatoxin-sensitive calcium currents in somata and axon terminals of rat supraoptic neurones.
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pubmed:affiliation |
Centre for Research in Neuroscience, McGill University, Montreal, Quebec, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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