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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1996-10-21
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pubmed:abstractText |
Classical swine fever virus (CSFV)-specific cytotoxic T lymphocytes (CTL) were derived from peripheral blood mononuclear leukocytes of immunized NIH-minipigs (MHC d/d haplotype) after in vitro restimulation with infectious CSFV. Their cytotoxic activity was determined against CSFV-infected target cells obtained from simian virus 40 (SV40) large T antigen-transfected immortalized kidney cells of a syngeneic miniature swine. Experiments with separated effector cell populations revealed that the CSFV-specific cytotoxic activity was mediated by CD4(-)CD6+CD8+ MHC class I-restricted T lymphocytes. Infection of target cells with various vaccinia virus/CSFV recombinants led to the identification of a major antigenic site for CSFV-specific CTL near the cleavage site between the non-structural proteins p80 (NS3) and p10 (NS4a). Using synthetic overlapping nonapeptides which covered this protein region the sequence ENALLVALF is the first sequence to be identified as an MHC class I-restricted T cell epitope recognized by CSFV-specific CTL.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/CD6 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Nonstructural Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1317
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
76 ( Pt 12)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3039-49
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8847509-Amino Acid Sequence,
pubmed-meshheading:8847509-Animals,
pubmed-meshheading:8847509-Antigens, CD,
pubmed-meshheading:8847509-Antigens, CD4,
pubmed-meshheading:8847509-Antigens, CD8,
pubmed-meshheading:8847509-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:8847509-Cell Line, Transformed,
pubmed-meshheading:8847509-Classical Swine Fever,
pubmed-meshheading:8847509-Classical swine fever virus,
pubmed-meshheading:8847509-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:8847509-Epitope Mapping,
pubmed-meshheading:8847509-Epitopes, T-Lymphocyte,
pubmed-meshheading:8847509-Molecular Sequence Data,
pubmed-meshheading:8847509-Peptides,
pubmed-meshheading:8847509-Swine,
pubmed-meshheading:8847509-Swine, Miniature,
pubmed-meshheading:8847509-T-Lymphocyte Subsets,
pubmed-meshheading:8847509-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:8847509-Vaccinia virus,
pubmed-meshheading:8847509-Viral Nonstructural Proteins
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pubmed:year |
1995
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pubmed:articleTitle |
Classical swine fever virus-specific cytotoxic T lymphocytes and identification of a T cell epitope.
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pubmed:affiliation |
Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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