Linked Life Data

8847449

Source:http://linkedlifedata.com/resource/pubmed/id/8847449

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-10-24
pubmed:abstractText
A sensitive sperm-motility test for the evaluation of cytotoxic effects of carbofuran and glyphosate in a defined protein-free culture medium is described. The sperm motility was compared to that obtained with a protein-containing medium. The use of protein-free medium considerably increased the sensitivity of sperm cells from rabbit and human to the toxic effects of the pesticide. The respective IC50 values (the concentration needed to cause 50% inhibition of sperm motility) in protein-free medium of carbofuran and glyphosate were 321 and 48.2 microM with human sperm, and 116 and 23.5 microM with rabbit sperm. Whereas, the corresponding values in protein-containing medium were 920 and 740 microM, and 910 and 500 microM with human and rabbit sperm, respectively. Our results show that testing human and rabbit sperm in protein-free medium proves to be a more sensitive method than that in protein-containing medium. Additionally, the use of rabbit sperm is a more sensitive test system than human sperm. This study suggests that the rabbit sperm test appears to have a potential for the assessment of toxicity on human reproduction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0360-1234
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
99-115
pubmed:dateRevised
2009-7-21
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
A sensitive sperm-motility test for the assessment of cytotoxic effect of pesticides.
pubmed:affiliation
Department of Environmental Studies, Alexandria University, Egypt.
pubmed:publicationType
Journal Article