Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-10-23
|
| pubmed:abstractText |
Memory T- and B-cell responses to trypanosome antigens were measured in peripheral blood mononuclear cells, spleen and lymph node cells obtained from four trypanotolerant N'Dama cattle which had been exposed to six experimental infections with Trypanosoma congolense. These cattle were treated with trypanocidal drugs following each infection and had remained aparasitemic for 3 years prior to this study. The antigens used were whole trypanosome lysate, variable surface glycoprotein, a 33-kDa cysteine protease (congopain) and a 70-kDa heat-shock protein. As parameters of T-cell-mediated immunity, we measured T-cell proliferation and IFN-gamma production. Lymph node cells, spleen cells and peripheral blood mononuclear cells all proliferated to a mitogenic stimulus (concanavalin A) but only lymph node cells responded to trypanosome antigens. Similarly, IFN-gamma was produced by both lymph node and spleen cells stimulated with concanavalin A but only by lymph node cells stimulated with variable surface glycoprotein and whole trypanosome lysate. T. congolense-specific antibodies were detected in sera and in supernatants of cultured lymph node and spleen cells after in vitro stimulation with lipopolysaccharide and recombinant bovine interleukin-2. In conclusion, we have demonstrated that memory T- and B-cell responses are detectable in various lymphoid organs in cattle 3 years following infection and treatment with T. congolense.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0165-2478
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
48
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
29-34
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8847087-Animals,
pubmed-meshheading:8847087-Antibodies, Protozoan,
pubmed-meshheading:8847087-Antigens, Protozoan,
pubmed-meshheading:8847087-B-Lymphocytes,
pubmed-meshheading:8847087-Cattle,
pubmed-meshheading:8847087-Immunization,
pubmed-meshheading:8847087-Immunologic Memory,
pubmed-meshheading:8847087-Interferon-gamma,
pubmed-meshheading:8847087-Leukocytes, Mononuclear,
pubmed-meshheading:8847087-Lymph Nodes,
pubmed-meshheading:8847087-Lymphocyte Activation,
pubmed-meshheading:8847087-Spleen,
pubmed-meshheading:8847087-T-Lymphocytes,
pubmed-meshheading:8847087-Trypanosoma congolense,
pubmed-meshheading:8847087-Trypanosomiasis, African,
pubmed-meshheading:8847087-Trypanosomiasis, Bovine
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Trypanosoma congolense: tissue distribution of long-term T- and B-cell responses in cattle.
|
| pubmed:affiliation |
International Livestock Research Institute, Nairobi, Kenya.
|
| pubmed:publicationType |
Journal Article
|