Linked Life Data

8844763

Source:http://linkedlifedata.com/resource/pubmed/id/8844763

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1997-2-19
pubmed:abstractText
We are investigating peptidergic neurotransmission between ganglia in the Aplysia CNS. Neurons in the cerebral ganglia of Aplysia synthesize [35S]methionine-labeled peptides, which are transported to other central ganglia by fast axonal transport. We report the characterization of one of these peptides, termed cerebral peptide 1 (CP1). Transported [35S]methionine-labeled CP1 was used as a probe for the purification of CP1 from the pooled extracts of 1000 cerebral ganglia using three sequential modes of RP-HPLC. Amino acid sequence analysis by automated Edman degradation yielded the following sequence: Phe-Ser-Gly-Leu-Met-Ser-Glu-Gly-Ser-Ser-Leu-Glu-Ala. This sequence was consistent with data from amino acid composition analysis and FAB-MS of the purified peptide. In addition, a synthetic peptide with the proposed sequence coeluted with [35S]methionine-labeled native CP1 using the three RP-HPLC conditions used in the purification. Antisera raised against synthetic CP1 stained about 90 neuronal cell bodies in the cerebral ganglion including those of the asymmetric H cluster. Extracts of the H cluster dissected from ganglia incubated in [35S]methionine synthesized a radiolabeled peptide that coeluted with synthetic CP1, indicating that these neurons indeed synthesize authentic CP1. The other major ganglia each contained only a few CP1-immunoreactive neurons. The neuropil of each ganglion contains both smooth and varicose-immunoreactive fibers. Thus, CP1 is broadly distributed within the Aplysia CNS and may be a new peptide transmitter.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0196-9781
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
753-61
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Purification, primary structure, and neuronal localization of cerebral peptide 1 from Aplysia.
pubmed:affiliation
Department of Pharmacological and Physiological Sciences, University of Chicago, IL 60637, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.