Linked Life Data

8843664

Source:http://linkedlifedata.com/resource/pubmed/id/8843664

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1996-12-2
pubmed:abstractText
The microscale techniques of CZE, cIEF and SDS capillary electrophoresis have been evaluated for the analysis of a complex glycoprotein, recombinant tissue plasminogen activator (rtPA). A series of omega-amino acid buffers (pH approximately 5) was found suitable for the CZE separation of rtPA on coated capillaries. rtPA could be resolved into a series of major and minor peaks in an epsilon-aminocaproic acid buffer containing 0.01% (v/v) Tween 80. For cIEF, a two step method with pressure mobilization was utilized. Using a commercial instrument, either a polymer solution with a 50 microns I.D. capillary or narrow bore capillaries without a polymer solution (25 microns I.D.) were employed. rtPA was resolved into at least eight species within a pI range of 6.4-9.2 using Ampholine 3.5-10. Migration time precision for the major peaks ranged from 0.2% for CZE to < or = 2-3% R.S.D. for cIEF. Total recovery of rtPA from the capillary was also demonstrated for both methods. Analysis of rtPA, rtPA Type I, rtPA Type II and the desialylated forms resulted in the expected elution profiles. Finally, the potential of SDS capillary electrophoresis using a coated capillary for an rtPA Type I/Type II purity assay was shown.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
744
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
155-65
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed-meshheading:8843664-Acetic Acids, pubmed-meshheading:8843664-Acrylamides, pubmed-meshheading:8843664-Adsorption, pubmed-meshheading:8843664-Amino Acid Sequence, pubmed-meshheading:8843664-Ampholyte Mixtures, pubmed-meshheading:8843664-Animals, pubmed-meshheading:8843664-Electrophoresis, pubmed-meshheading:8843664-Electrophoresis, Capillary, pubmed-meshheading:8843664-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:8843664-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:8843664-Goats, pubmed-meshheading:8843664-Immune Sera, pubmed-meshheading:8843664-Isoelectric Focusing, pubmed-meshheading:8843664-Isoelectric Point, pubmed-meshheading:8843664-Molecular Sequence Data, pubmed-meshheading:8843664-Neuraminidase, pubmed-meshheading:8843664-Plasminogen, pubmed-meshheading:8843664-Polyvinyl Alcohol, pubmed-meshheading:8843664-Protein Structure, Secondary, pubmed-meshheading:8843664-Recombinant Proteins, pubmed-meshheading:8843664-Reproducibility of Results, pubmed-meshheading:8843664-Sodium Dodecyl Sulfate, pubmed-meshheading:8843664-Surface-Active Agents, pubmed-meshheading:8843664-Tissue Plasminogen Activator
pubmed:year
1996
pubmed:articleTitle
Examination of capillary zone electrophoresis, capillary isoelectric focusing and sodium dodecyl sulfate capillary electrophoresis for the analysis of recombinant tissue plasminogen activator.
pubmed:affiliation
Northeastern University, Barnett Institute, Boston, MA, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.