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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
34
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pubmed:dateCreated |
1996-12-18
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pubmed:abstractText |
The rate of cleavage of a 13-mer substrate by an all-RNA ribozyme with 2 10-mer hybridising arms (TAT RA), appears to be limited by a conformational change involving the longer than necessary arms. The same limitation does not apply to a similar ribozyme with DNA arms (TAT RB) or to an all-RNA ribozyme with only 6 hybridising bases on each arm (TAT RA 6x2). The limitation does not arise in the hybridisation step nor in the dissociation of products. An extra step involving a conformational change of the pre-formed ribozyme-substrate prior to cleavage is introduced to explain the observed kinetics.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0261-3166
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
217-8
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pubmed:dateRevised |
2000-12-18
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pubmed:meshHeading |
pubmed-meshheading:8841629-Base Sequence,
pubmed-meshheading:8841629-DNA,
pubmed-meshheading:8841629-Hydrogen-Ion Concentration,
pubmed-meshheading:8841629-Kinetics,
pubmed-meshheading:8841629-Models, Biological,
pubmed-meshheading:8841629-Nucleic Acid Conformation,
pubmed-meshheading:8841629-Oligodeoxyribonucleotides,
pubmed-meshheading:8841629-Oligoribonucleotides,
pubmed-meshheading:8841629-RNA,
pubmed-meshheading:8841629-RNA, Catalytic,
pubmed-meshheading:8841629-Substrate Specificity
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pubmed:year |
1995
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pubmed:articleTitle |
The mechanism of the hammerhead ribozyme.
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pubmed:affiliation |
CSIRO, Division of Biomolecular Engineering, North Ryde, NSW, Australia.
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pubmed:publicationType |
Journal Article
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