Linked Life Data

8841453

Source:http://linkedlifedata.com/resource/pubmed/id/8841453

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-12-16
pubmed:abstractText
GlyCAM-1 is an endothelial-derived mucin found in the conditioned medium of organ-cultured lymph nodes and in serum. It is a ligand for L-selectin, but also interacts with P and E-selectin. It may play a role in the modulation of leukocyte trafficking into secondary lymphoid tissues as well as chronic inflammatory sites. Here we report the development of an ELISA that quantifies GlyCAM-1 in complex biological fluids such as serum. The ELISA response is linear over a concentration range of 13-800 ng/ml. The level of GlyCAM-1 in mouse serum was found to be 1.3-1.6 micrograms/ml. Using mouse serum as starting material, we report a rapid and efficient purification scheme for the isolation of biologically active GlyCAM-1. This material may now be used to address the biological functions of GlyCAM-1 and to further define its structural features.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
196
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
153-61
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Purification and quantification of L-selectin-reactive GlyCAM-1 from mouse serum.
pubmed:affiliation
Department of Anatomy, University of California, San Francisco 94143-0452, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.