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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1997-1-9
|
| pubmed:abstractText |
To characterize the functional coupling of the beta 2-AR to the cardiac Ca2+ channel in a system with a single receptor subtype, we stably cotransfected a Chinese hamster fibroblast (CHW) cell line, which lacks beta 2-ARs and Ca2+ channels, with the rabbit cardiac Ca2+ channel alpha 1 and beta 2 subunits and the human beta 2-AR cDNAs. The effects of beta 2-AR stimulation on the expressed Ca2+ channel current were examined using the whole-cell patch-clamp technique. CHW cells transfected with the Ca2+ channel subunits displayed a voltage-dependent inward current having properties typical of native cardiac L-type Ca2+ channels. The expressed current was increased by a phosphorylation-dependent mechanism. CHW cells cotransfected with the Ca2+ channel subunits and the beta 2-AR were responsive to isoproterenol (Iso) in a dose-dependent manner. Iso (10 microM) increased peak Ca2+ channel current to 172 +/- 5% (n = 17) of control amplitude, indicating that the expressed Ca2+ channels are functionally coupled to the beta 2-AR. The results demonstrate unequivocally that beta 2-ARs can modulate the activity of cardiac Ca2+ channels, independent of beta 1-ARs. The results also demonstrate the usefulness of the CHW heterologous expression system, the first to reconstitute physiological modulation of an L-type Ca2+ channel by the beta 2-AR, for studying receptor subtype-specific regulation of the Ca2+ channel.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic beta-Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels, L-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Isoproterenol,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic, beta-2
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
1052-8040
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
5
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
219-31
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8840400-Adrenergic beta-Agonists,
pubmed-meshheading:8840400-Animals,
pubmed-meshheading:8840400-Calcium Channels,
pubmed-meshheading:8840400-Calcium Channels, L-Type,
pubmed-meshheading:8840400-Cell Line,
pubmed-meshheading:8840400-Cricetinae,
pubmed-meshheading:8840400-Cricetulus,
pubmed-meshheading:8840400-Cyclic AMP,
pubmed-meshheading:8840400-Electrophysiology,
pubmed-meshheading:8840400-Fibroblasts,
pubmed-meshheading:8840400-Gene Expression,
pubmed-meshheading:8840400-Humans,
pubmed-meshheading:8840400-Isoproterenol,
pubmed-meshheading:8840400-Myocardium,
pubmed-meshheading:8840400-Phosphorylation,
pubmed-meshheading:8840400-Rabbits,
pubmed-meshheading:8840400-Receptors, Adrenergic, beta-2,
pubmed-meshheading:8840400-Transfection
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Beta 2-adrenergic receptor regulation of the cardiac L-type Ca2+ channel coexpressed in a fibroblast cell line.
|
| pubmed:affiliation |
Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, OH 45267, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|