8839846

Source:http://linkedlifedata.com/resource/pubmed/id/8839846

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0007589, umls-concept:C0011306, umls-concept:C0018956, umls-concept:C0023005, umls-concept:C0230694, umls-concept:C0449475, umls-concept:C1332710, umls-concept:C1511938, umls-concept:C1515021, umls-concept:C1522445, umls-concept:C1533691
pubmed:issue	7
pubmed:dateCreated	1996-11-7
pubmed:abstractText	We have demonstrated recently that Birbeck granule-positive Langerhans cells (LC) can be derived from CD34+ peripheral blood progenitor cells in the presence of a seven-cytokine cocktail (CC7-7). Here, we show that the sequential use of early-acting hematopoietic growth factors, stem cell factor, interleukin (IL)-3, and IL-6, followed on day 8 by differentiation in the two-factor combination IL-4 plus granulocytemacrophage colony-stimulating factor (GM-CSF) (CC4GM) is more efficient and allows the cells to be arrested in the LC stage for more than 1 week while continuous maturation occurs in CC7-7. Maturation of LC to interdigitating dendritic cells (DC) could specifically be induced within 60 hours by addition of tumor necrosis factor-alpha (20 ng/mL) or lipopolysaccharide (100 ng/mL). Using LC that had been enriched to greater than 90% CD1a+ cells by an immunoaffinity column, we were able to define clear-cut differences between LC and DC that corroborate data of the respective cells derived from epithelial borders (LC) or from lymph nodes (LN) and spleen (DC). Thus, molecules and functions involved in antigen (AG) uptake and processing were highly expressed in LC, while those involved in AG presentation were at maximum in DC. LC were CD1a+2 DR+2, CD23+, CD36+, CD80-, CD86-, and CD25-, while DC were CD1a+/- DR+3, CD23-, CD36-, CD80+, CD86+2, and CD25+, CD40 and CD32 were moderately expressed and nearly unchanged on maturation, in contrast to monocyte-derived DC. Macropinocytosis of fluorescein isothiocyanate-dextran was dominant in LC, as were multilamellar major histocompatibility complex (MHC) class II compartments (MIICs), which were detected by electron microscopy. The functional dichotomy of these cell types was finally supported by testing the AG-presenting cell function for tetanus toxoid to primed autologous T-cell lines, which was optimal when cells were loaded with AG as LC and subsequently induced to become DC.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/7603509
pubmed:citationSubset	AIM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34, http://linkedlifedata.com/resource/pubmed/chemical/Cisplatin, http://linkedlifedata.com/resource/pubmed/chemical/Epirubicin, http://linkedlifedata.com/resource/pubmed/chemical/Etoposide, http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens, http://linkedlifedata.com/resource/pubmed/chemical/Ifosfamide, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-3, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Stem Cell Factor, http://linkedlifedata.com/resource/pubmed/chemical/Tetanus Toxoid, http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
pubmed:status	MEDLINE
pubmed:month	Oct
pubmed:issn	0006-4971
pubmed:author	pubmed-author:FischPP, pubmed-author:HerbstBB, pubmed-author:KöhlerGG, pubmed-author:KulmburgPP, pubmed-author:LindemannAA, pubmed-author:MackensenAA, pubmed-author:MertelsmannRR, pubmed-author:RosenthalF MFM, pubmed-author:SchaeferH EHE, pubmed-author:VeelkenHH
pubmed:issnType	Print
pubmed:day	1
pubmed:volume	88
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	2541-8
pubmed:dateRevised	2006-11-15
pubmed:meshHeading	pubmed-meshheading:8839846-Antigen Presentation, pubmed-meshheading:8839846-Antigens, CD, pubmed-meshheading:8839846-Antigens, CD34, pubmed-meshheading:8839846-Bone Marrow, pubmed-meshheading:8839846-Cell Differentiation, pubmed-meshheading:8839846-Cells, Cultured, pubmed-meshheading:8839846-Cisplatin, pubmed-meshheading:8839846-Cytoplasmic Granules, pubmed-meshheading:8839846-Dendritic Cells, pubmed-meshheading:8839846-Drug Synergism, pubmed-meshheading:8839846-Epirubicin, pubmed-meshheading:8839846-Epithelial Cells, pubmed-meshheading:8839846-Etoposide, pubmed-meshheading:8839846-HLA-DR Antigens, pubmed-meshheading:8839846-Hematopoietic Stem Cells, pubmed-meshheading:8839846-Humans, pubmed-meshheading:8839846-Ifosfamide, pubmed-meshheading:8839846-Immunophenotyping, pubmed-meshheading:8839846-Interleukin-3, pubmed-meshheading:8839846-Interleukin-4, pubmed-meshheading:8839846-Interleukin-6, pubmed-meshheading:8839846-Langerhans Cells, pubmed-meshheading:8839846-Lymph Nodes, pubmed-meshheading:8839846-Recombinant Proteins, pubmed-meshheading:8839846-Spleen, pubmed-meshheading:8839846-Stem Cell Factor, pubmed-meshheading:8839846-T-Lymphocyte Subsets, pubmed-meshheading:8839846-Tetanus Toxoid, pubmed-meshheading:8839846-Tumor Necrosis Factor-alpha
pubmed:year	1996
pubmed:articleTitle	In vitro differentiation of CD34+ hematopoietic progenitor cells toward distinct dendritic cell subsets of the birbeck granule and MIIC-positive Langerhans cell and the interdigitating dendritic cell type.
pubmed:affiliation	Department of Medicine I (Hematology/Oncology), University Medical Center Freiburg, Germany.
pubmed:publicationType	Journal Article, Comparative Study, Research Support, Non-U.S. Gov't