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rdf:type |
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lifeskim:mentions |
umls-concept:C0008013,
umls-concept:C0015388,
umls-concept:C0021368,
umls-concept:C0027950,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0086045,
umls-concept:C0392747,
umls-concept:C0443172,
umls-concept:C0443199,
umls-concept:C1707169
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pubmed:issue |
3
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pubmed:dateCreated |
1997-5-28
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pubmed:abstractText |
Recently we have purified four neutrophil chemotactic factors from conditioned medium of rat granulation tissue. Two chemokines besides cytokine-induced neutrophil chemoattractant-1 (CINC-1, formerly called CINC) and CNC-3/macrophage inflammatory protein-2 (MIP-2) were novel chemoattractants, designated as CINC-2 alpha and CINC-2 beta. In the present report, we developed an enzyme-linked immunosorbent assay (ELISA) specific for CINC-2. The biotin-streptavidin sandwich ELISA for CINC-2 beta detected CINC-2 alpha and CINC- 2 beta equally well between 1 ug/mI and 300 ng/ml, but did not show cross-reactivity with CINC-1 and CINC-3. The concentrations of CINC-1 and CINC-2 in the exudate during rat lipopolysaccharide (LPS)-induced inflammation were determined. The CINC-1 concentration in the exudate reached a maximum at 4 h after LPS injection, whereas the CINC-2 level steadily increased up to 8 h. The number of infiltrated cells in the exudate increased linearly until 6 h and gradually up to 8 h. Increase in the cell number was correlated with total concentrations of CINC-1 and CINC-2. The results suggest that CINC-2 as well as CINC-1 plays an important role in accumulation of neutrophils into the inflammatory lesion of LPS- induced inflammation in rats.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL1,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL2,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Cxcl1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cxcl2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Gm1960 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1043-4666
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
222-6
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8833037-Animals,
pubmed-meshheading:8833037-Chemokine CXCL1,
pubmed-meshheading:8833037-Chemokine CXCL2,
pubmed-meshheading:8833037-Chemokines,
pubmed-meshheading:8833037-Chemokines, CXC,
pubmed-meshheading:8833037-Chemotactic Factors,
pubmed-meshheading:8833037-Chromatography, Affinity,
pubmed-meshheading:8833037-Cross Reactions,
pubmed-meshheading:8833037-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:8833037-Escherichia coli,
pubmed-meshheading:8833037-Granuloma,
pubmed-meshheading:8833037-Growth Substances,
pubmed-meshheading:8833037-Inflammation,
pubmed-meshheading:8833037-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:8833037-Lipopolysaccharides,
pubmed-meshheading:8833037-Male,
pubmed-meshheading:8833037-Neutrophils,
pubmed-meshheading:8833037-Rabbits,
pubmed-meshheading:8833037-Rats,
pubmed-meshheading:8833037-Rats, Wistar,
pubmed-meshheading:8833037-Recombinant Fusion Proteins
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pubmed:year |
1996
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pubmed:articleTitle |
Differential changes in the concentrations of cytokine-induced neutrophil chemoattractant (CINC)-1 and CINC-2 in exudate during rat lipopolysaccharide-induced inflammation.
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pubmed:affiliation |
Department of Physiological Chemistry, Toyama Medical and Pharmaceutical University, Sugitani, Toyana, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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