Linked Life Data

8832907

Source:http://linkedlifedata.com/resource/pubmed/id/8832907

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-5-22
pubmed:abstractText
Extensive metabolizers of drugs which are oxidized by cytochrome P450s show wide interindividual variability in drug metabolism. Elucidation of the relationship between specific P450 isozyme mRNA expression and variability in metabolism of specific drugs could improve understanding of the molecular mechanism involved. In this study, a novel non-radiometric reverse transcription polymerase chain reaction based assay was used to quantify CYP2D6 mRNA levels in peripheral blood mononuclear cells in twelve human volunteers who were phenotyped as extensive metabolizers of debrisoquine. We report a significant correlation (r=0.724, p= 0.008) between CYP2D6 mRNA levels in circulating peripheral blood mononuclear cells and the in vivo debrisoquine recovery ratio. This suggests that interindividual variability in extensive metabolizers could be related, in part, to differences in gene expression. Circulating peripheral blood mononuclear cells can provide an accessible ex vivo measure of this phenomenon.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1078-0297
pubmed:author
pubmed:issnType
Print
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
149-159
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
CYP2D6 mRNA expression in circulating peripheral blood mononuclear cells correlates with in vivo debrisoquine hydroxylase activity in extensive metabolizers.
pubmed:affiliation
Department of Anesthesiology, Graduate School of Public Health, University of Pittsburgh, PA, USA.
pubmed:publicationType
Journal Article, Clinical Trial, Research Support, Non-U.S. Gov't