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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1996-10-16
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pubmed:abstractText |
Infection with JHMV results in the transcriptional activation of two host cell genes encoding proinflammatory cytokines, tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta. Analysis of irradiated mice showed that IL-1 beta mRNA accumulation in the central nervous system was predominantly derived from the mononuclear infiltrate. By contrast, accumulation of TNF-alpha mRNA was unaffected by immunosuppression, suggesting that resident cells were the source of this cytokine. Infected mice were treated with anti-TNF antibody to determine if TNF-alpha contributed to either the encephalomyelitis or demyelination associated with JHMV infection. Surprisingly, neither the cellular infiltrate nor demyelination were affected. In vitro analysis showed that IL-1 beta but not TNF was secreted from JHMV infected macrophages. The absence of TNF secretion is due to a block in translation of the TNF mRNA which accumulates during infection.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0065-2598
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
380
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
173-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8830475-Animals,
pubmed-meshheading:8830475-Brain,
pubmed-meshheading:8830475-Cells, Cultured,
pubmed-meshheading:8830475-Coronavirus Infections,
pubmed-meshheading:8830475-Cytokines,
pubmed-meshheading:8830475-Demyelinating Diseases,
pubmed-meshheading:8830475-Encephalomyelitis,
pubmed-meshheading:8830475-Gene Expression Regulation, Viral,
pubmed-meshheading:8830475-Interleukin-1,
pubmed-meshheading:8830475-Kinetics,
pubmed-meshheading:8830475-Lipopolysaccharides,
pubmed-meshheading:8830475-Macrophages, Peritoneal,
pubmed-meshheading:8830475-Mice,
pubmed-meshheading:8830475-Mice, Inbred BALB C,
pubmed-meshheading:8830475-Murine hepatitis virus,
pubmed-meshheading:8830475-Protein Biosynthesis,
pubmed-meshheading:8830475-RNA, Messenger,
pubmed-meshheading:8830475-Time Factors,
pubmed-meshheading:8830475-Transcription, Genetic,
pubmed-meshheading:8830475-Tumor Necrosis Factor-alpha
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pubmed:year |
1995
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pubmed:articleTitle |
Transcription and translation of proinflammatory cytokines following JHMV infection.
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pubmed:affiliation |
Department of Microbiology, University of Southern California, Los Angeles, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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