Linked Life Data

8830470

Source:http://linkedlifedata.com/resource/pubmed/id/8830470

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1996-10-16
pubmed:abstractText
A virus inducing a cytopathic effect on porcine alveolar macrophages was isolated from the lungs of a pig with respiratory problems and lesions of proliferative and necrotizing pneumonia. The isolate was found to react with porcine reproductive and respiratory syndrome virus (PRRSV) monoclonal antibodies by indirect immunofluorescence and was designated LHVA-93-3. The virus could also be propagated on the MARC-145 cell line. The LHVA-93-3 macrophage-passaged isolate was inoculated orally or intranasally in four-week-old specific pathogen-free pigs. Histologically, focal to multifocal lesions of proliferative, necrotizing and interstitial pneumonia could be observed in the lungs of pigs inoculated orally or intranasally, 6 and 10 days post-inoculation. Virus could be reisolated from essentially the same tissues including serum following both routes of infection. The distribution of PRRSV antigens in fixed tissues as determined by immunogold silver staining (IGSS) was similar in orally or intranasally inoculated pigs. The results of this experimental transmission study indicate that pigs may become infected by PRRSV following oral as well as intranasal exposure.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0065-2598
pubmed:author
pubmed:issnType
Print
pubmed:volume
380
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
139-44
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Isolation and experimental oral transmission in pigs of a porcine reproductive and respiratory syndrome virus isolate.
pubmed:affiliation
Laboratoire d'Hygiène Vétérinaire et Alimentaire, Agriculture Canada, Saint-Hyacinthe, Québec, Canada.
pubmed:publicationType
Journal Article