Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-10-16
pubmed:databankReference
pubmed:abstractText
Rat milk and digestive juices contain transferrin but not lactoferrin, which is a major iron-binding protein in these secretions of human and mouse. To compare the structure of rat transferrin to that of transferrins and lactoferrins in other species, we isolated a cDNA clone containing the entire coding region of transferrin from rat liver and determined its sequence. The amino-acid sequence of rat transferrin had 69.8% identity with that of human transferrin and 48.8% identity with that of human lactoferrin. Rat transferrin, like other transferrins, had the potential N-linked glycosylation site only in the C-terminal domain, although lactoferrins characterized so far contained the glycosylation sites in both the N- and C-terminal domains. Southern and Northern analyses showed that there was the gene specifically hybridized with the mouse lactoferrin cDNA in rat genomic DNA, but only the transferrin mRNA was detected in mammary gland, submaxillary gland and pancreas of rat. These results suggest that the rat lactoferrin gene is silent in the mammary gland, and transferrin can serve as a functional substitute for lactoferrin in rat.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1096-4959
pubmed:author
pubmed:issnType
Print
pubmed:volume
113
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
491-7
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:8829802-Amino Acid Sequence, pubmed-meshheading:8829802-Animals, pubmed-meshheading:8829802-Base Sequence, pubmed-meshheading:8829802-Cloning, Molecular, pubmed-meshheading:8829802-Consensus Sequence, pubmed-meshheading:8829802-DNA, Complementary, pubmed-meshheading:8829802-Female, pubmed-meshheading:8829802-Humans, pubmed-meshheading:8829802-Lactation, pubmed-meshheading:8829802-Lactoferrin, pubmed-meshheading:8829802-Liver, pubmed-meshheading:8829802-Male, pubmed-meshheading:8829802-Mammary Glands, Animal, pubmed-meshheading:8829802-Mice, pubmed-meshheading:8829802-Mice, Inbred Strains, pubmed-meshheading:8829802-Molecular Sequence Data, pubmed-meshheading:8829802-Pancreas, pubmed-meshheading:8829802-Rabbits, pubmed-meshheading:8829802-Rats, pubmed-meshheading:8829802-Rats, Wistar, pubmed-meshheading:8829802-Sequence Homology, Amino Acid, pubmed-meshheading:8829802-Submandibular Gland, pubmed-meshheading:8829802-Transferrin
pubmed:year
1996
pubmed:articleTitle
Complete sequence analysis of rat transferrin and expression of transferrin but not lactoferrin in the digestive glands.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo, Japan.
pubmed:publicationType
Journal Article, Comparative Study