Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-12-6
|
| pubmed:abstractText |
We examined the functional properties of central nicotinic acetylcholine receptors at the single-channel level using tight-seal, voltage-clamp techniques. Single-channel currents were recorded from cell-attached patches on lateral spiriform neurons in chick brain slices. These neurons are known to express functional nicotinic receptors that are insensitive to the antagonists alpha-bungarotoxin and kappa-bungarotoxin, and which exhibit a high affinity for nicotine and other nicotinic agonists. Single-channel openings were observed in 84% of patches (n = 118) when the nicotinic agonists acetylcholine (1-100 microM), carbamylcholine (3-100 microM), or nicotine (3-10 microM) were present in the patch pipette. In contrast, single-channels were markedly reduced in number or entirely absent when the nicotinic antagonist dihydro-beta-erythroidine was present along with acetylcholine (n = 7) or when no agonist was present in the pipette (n = 22). Single-channel openings displayed inward rectification at depolarized potentials, and were dependent on extracellular sodium. Between 1 and 30 microM acetylcholine, a dose-response relationship was observed between agonist concentration and single-channel open probability during the first minute following seal formation. Multiple classes of single nicotinic channels, with calculated mean slope conductances of 15, 31, 40, and approximately 70 pS, were observed in membrane patches on different neurons within the lateral spiriform nucleus, and even within single patches on individual neurons. We conclude that neurons within the lateral spiriform nucleus express functionally heterogeneous nicotinic receptors and that in some neurons different nicotinic receptor subtypes are present in close proximity to each other on the same cell surface.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0006-8993
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
24
|
| pubmed:volume |
725
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
95-105
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading | |
| pubmed:year |
1996
|
| pubmed:articleTitle |
Single-channel recording in brain slices reveals heterogeneity of nicotinic receptors on individual neurons within the chick lateral spiriform nucleus.
|
| pubmed:affiliation |
Department of Pharmacological and Physiological Science, St. Louis University School of Medicine, MO 63104, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|