Linked Life Data

8825068

Source:http://linkedlifedata.com/resource/pubmed/id/8825068

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1996-12-5
pubmed:abstractText
Fusion regulatory proteins (FRPs) regulate virus-mediated cell fusion and multinucleated giant cell formation of monocytes. Anti-FRP-1 mAbs immunoprecipitated 80 kDa and 38 kDa proteins from HeLa cells. After long exposure other bands were detected, suggesting the presence of molecule(s) associated with FRP-1. To identify the molecule(s), we prepared monoclonal antibodies against immunoaffinity-purified FRP-1 complex derived from membrane fractions of HeLa cells. Immunofluorescence microscopy revealed that these monoclonal antibodies recognized the intracytoplasmic molecules in HeLa cells. Using immunoblotting, the antibodies reacted with 200 kDa, 70 kDa, 55 kDa and 35 kDa molecules, so we designated these molecules as FRP-related molecules (FRMs). Subsequently, we performed gene cloning from a HeLa lambda gt11 cDNA library using anti-FRM mAbs and immunoblotting analysis with either purified cytoskeletal proteins or specific antibodies against various cytoskeletal proteins. Three kinds of positive clone were obtained, which encoded partial sequences of vimentin, tropomyosin, and heat shock cognate protein 70 (hsc70). The 200 kDa molecule was expected to be a myosin heavy chain, judging from the immunoblotting pattern. Immunoblotting confirmed that these purified proteins were readily recognized by anti-FRM mAbs. Furthermore, anti-vimentin and anti-myosin mAbs reacted with the precipitates by anti-FRP-1 mAb, indicating a physical association between FRP-1 molecules and these cytoskeletal proteins. When anti-FRP-1 mAb was added to culture fluids of HeLa cells, the cell-shape and immunofluorescence-pattern stained with anti-FRM mAbs changed. Taken together, the fusion regulatory molecular complex is suggested to consist of at least FRP-1, hsc70, actomyosin and vimentin systems.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0386-7196
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
473-83
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:8825068-Antibodies, Monoclonal, pubmed-meshheading:8825068-Antigens, CD, pubmed-meshheading:8825068-Antigens, CD98, pubmed-meshheading:8825068-Carrier Proteins, pubmed-meshheading:8825068-Cell Fusion, pubmed-meshheading:8825068-Cell Line, pubmed-meshheading:8825068-Cell Membrane, pubmed-meshheading:8825068-Cytoskeletal Proteins, pubmed-meshheading:8825068-DNA, Complementary, pubmed-meshheading:8825068-Giant Cells, pubmed-meshheading:8825068-HIV, pubmed-meshheading:8825068-HSP70 Heat-Shock Proteins, pubmed-meshheading:8825068-HeLa Cells, pubmed-meshheading:8825068-Humans, pubmed-meshheading:8825068-Immunoblotting, pubmed-meshheading:8825068-Monocytes, pubmed-meshheading:8825068-Myosins, pubmed-meshheading:8825068-Precipitin Tests, pubmed-meshheading:8825068-Tropomyosin, pubmed-meshheading:8825068-Tumor Cells, Cultured, pubmed-meshheading:8825068-Vimentin
pubmed:year
1995
pubmed:articleTitle
Identification of fusion regulatory protein (FRP)-1/4F2 related molecules: cytoskeletal proteins are associated with FRP-1 molecules that regulate multinucleated giant cell formation of monocytes and HIV-induced cell fusion.
pubmed:affiliation
Department of Microbiology, Mie University School of Medicine, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't