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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1996-12-5
|
| pubmed:abstractText |
We have used a primary cloning assay to determine the frequency of 6-thioguanine (TG)-resistant tubular epithelial cells in kidney tissue from 72 human donors ranging in age from 2 to 94 years. The frequency of TG-resistant mutants ranged from approximately 5 x 10(-5) for donors in the first decade of life to approximately 2.5 x 10(-4) for donors in the eighth and later decades of life. Two different statistical analyses indicated that this increase in mutant frequency is exponential with age. We also observed a 2-fold higher TG-resistant mutant frequency in nephrectomy kidneys containing a coincident renal carcinoma. DNA sequence analyses revealed HPRT gene mutations in each of 14 TG-resistant mutants from seven unrelated donors. Thirteen of these 14 mutants resulted from independent mutational events. These results suggest that somatic mutations are common in renal--and perhaps in other human--epithelia, and thus could play an important role in the genesis of age-associated disease.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0964-6906
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
5
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
215-21
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8824877-Adolescent,
pubmed-meshheading:8824877-Adult,
pubmed-meshheading:8824877-Aged,
pubmed-meshheading:8824877-Aged, 80 and over,
pubmed-meshheading:8824877-Aging,
pubmed-meshheading:8824877-Base Sequence,
pubmed-meshheading:8824877-Carcinoma,
pubmed-meshheading:8824877-Cells, Cultured,
pubmed-meshheading:8824877-Child,
pubmed-meshheading:8824877-Child, Preschool,
pubmed-meshheading:8824877-DNA Primers,
pubmed-meshheading:8824877-Epithelium,
pubmed-meshheading:8824877-Humans,
pubmed-meshheading:8824877-Hypoxanthine Phosphoribosyltransferase,
pubmed-meshheading:8824877-Kidney,
pubmed-meshheading:8824877-Kidney Cortex,
pubmed-meshheading:8824877-Kidney Neoplasms,
pubmed-meshheading:8824877-Middle Aged,
pubmed-meshheading:8824877-Molecular Sequence Data,
pubmed-meshheading:8824877-Mutation,
pubmed-meshheading:8824877-Nephrectomy,
pubmed-meshheading:8824877-Thioguanine
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Somatic mutations are frequent and increase with age in human kidney epithelial cells.
|
| pubmed:affiliation |
Department of Pathology, University of Washington, Seattle 98195, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|