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rdf:type |
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lifeskim:mentions |
umls-concept:C0220905,
umls-concept:C0439855,
umls-concept:C0919490,
umls-concept:C1446165,
umls-concept:C1514562,
umls-concept:C1521761,
umls-concept:C1522492,
umls-concept:C1540067,
umls-concept:C1549781,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
18
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pubmed:dateCreated |
1996-12-13
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pubmed:abstractText |
Pip is a lymphoid-restricted IRF transcription factor that is recruited to composite elements within immunoglobulin light-chain gene enhancers through a specific interaction with the Ets factor PU.1. We have examined the transcriptional regulatory properties of Pip as well as the requirements for its interaction with PU.1 and DNA to form a ternary complex. We demonstrate that Pip is a dichotomous regulator; it specifically stimulates transcription in conjunction with PU.1, but represses alpha/beta-interferon-inducible transcription in the absence of PU.1. Thus, during B-cell activation and differentiation, Pip may function both as an activator to promote B cell-specific gene expression and as a repressor to inhibit the antiproliferative effects of alpha/beta-interferons. Mutational analysis of Pip reveals a carboxy-terminal segment that is important for autoinhibition of DNA binding and ternary complex formation. A domain of Pip containing this segment confers autoinhibition and PU.1-dependent binding activity to the DNA-binding domain of the related IRF family member, p48. On the basis of these and other data we propose a model for PU.1/Pip ternary complex formation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon Regulatory Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-Stimulated Gene Factor 3,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-Stimulated Gene Factor...,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Isgf3g protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/interferon regulatory factor-4,
http://linkedlifedata.com/resource/pubmed/chemical/interferon regulatory factor-8,
http://linkedlifedata.com/resource/pubmed/chemical/proto-oncogene protein Spi-1
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0890-9369
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2335-47
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8824592-Animals,
pubmed-meshheading:8824592-B-Lymphocytes,
pubmed-meshheading:8824592-Binding Sites,
pubmed-meshheading:8824592-Carrier Proteins,
pubmed-meshheading:8824592-DNA-Binding Proteins,
pubmed-meshheading:8824592-Gene Expression Regulation,
pubmed-meshheading:8824592-Interferon Regulatory Factors,
pubmed-meshheading:8824592-Interferon-Stimulated Gene Factor 3,
pubmed-meshheading:8824592-Interferon-Stimulated Gene Factor 3, gamma Subunit,
pubmed-meshheading:8824592-Interferon-alpha,
pubmed-meshheading:8824592-Interferon-gamma,
pubmed-meshheading:8824592-Mice,
pubmed-meshheading:8824592-Models, Molecular,
pubmed-meshheading:8824592-Nucleic Acid Conformation,
pubmed-meshheading:8824592-Protein Conformation,
pubmed-meshheading:8824592-Proto-Oncogene Proteins,
pubmed-meshheading:8824592-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8824592-Repressor Proteins,
pubmed-meshheading:8824592-Trans-Activators,
pubmed-meshheading:8824592-Transcription, Genetic,
pubmed-meshheading:8824592-Transcription Factors
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pubmed:year |
1996
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pubmed:articleTitle |
Pip, a lymphoid-restricted IRF, contains a regulatory domain that is important for autoinhibition and ternary complex formation with the Ets factor PU.1.
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pubmed:affiliation |
Howard Hughes Medical Institute, The University of Chicago, Illinois 60637, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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