Linked Life Data

8821944

Source:http://linkedlifedata.com/resource/pubmed/id/8821944

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-2-25
pubmed:abstractText
The AbrB protein of Bacillus subtilis regulates expression of numerous genes, primarily through specific binding interactions to DNA regions containing transcriptional promoters. Although over 15 target regions for AbrB binding to chromosomally located sequences have been analysed by DNase I footprinting, no obvious consensus sequence or motif has yet emerged from their examination. Using in vitro selection techniques, we have isolated optimal AbrB-binding sites from oligonucleotides containing 22 or 44 random base pairs. The best of these sites have an apparent in vitro Kd which is fivefold lower than a similar-sized DNA fragment containing the sequence corresponding to the AbrB-binding site on the spo0E gene. We tested one of the sites in vivo and found that it confers AbrB-mediated control upon a promoter not normally regulated by AbrB. In each of four separate trials, the selected sites possess motifs that converge to a simple consensus. It is argued that the nature and spacing of these motifs produce a type of three-dimensional DNA structure recognizable by AbrB, and that known in vivo sites, which lack these motifs, possess an approximation of the optimal structural determinant.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0950-382X
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
145-58
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8821944-Amino Acid Sequence, pubmed-meshheading:8821944-Bacillus subtilis, pubmed-meshheading:8821944-Bacterial Proteins, pubmed-meshheading:8821944-Base Sequence, pubmed-meshheading:8821944-Binding Sites, pubmed-meshheading:8821944-Consensus Sequence, pubmed-meshheading:8821944-DNA Footprinting, pubmed-meshheading:8821944-DNA-Binding Proteins, pubmed-meshheading:8821944-Gene Expression Regulation, Bacterial, pubmed-meshheading:8821944-Genes, Reporter, pubmed-meshheading:8821944-Molecular Sequence Data, pubmed-meshheading:8821944-Nucleic Acid Conformation, pubmed-meshheading:8821944-Oligodeoxyribonucleotides, pubmed-meshheading:8821944-Promoter Regions, Genetic, pubmed-meshheading:8821944-Recombinant Fusion Proteins, pubmed-meshheading:8821944-Sequence Alignment, pubmed-meshheading:8821944-Transcription Factors
pubmed:year
1996
pubmed:articleTitle
In vitro selection of optimal AbrB-binding sites: comparison to known in vivo sites indicates flexibility in AbrB binding and recognition of three-dimensional DNA structures.
pubmed:affiliation
Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't