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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1996-11-22
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pubmed:abstractText |
A liquid chromatographic system with a co-immobilized leucine dehydrogenase-NADH oxidase reactor is described for the determination of branched-chain amino acids such as I-leucine, I-isoleucine and I-valine. The enzymes were simultaneously immobilized on tresylate-containing poly(vinyl alcohol) beads. The separation was achieved by means of an ODS column with elution with phosphate buffer (pH 7.5). The hydrogen peroxide produced was detected chemiluminometrically via a luminol-hexacyanoferrate(III) reaction. The system gave a linear response from 0.3 to 300 mu M for each amino acid and the detection limit was 0.1 mu M.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9673
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
724
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
355-7
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8819797-Amino Acid Oxidoreductases,
pubmed-meshheading:8819797-Amino Acids, Branched-Chain,
pubmed-meshheading:8819797-Chromatography, High Pressure Liquid,
pubmed-meshheading:8819797-Enzymes, Immobilized,
pubmed-meshheading:8819797-Leucine Dehydrogenase,
pubmed-meshheading:8819797-Luminescent Measurements,
pubmed-meshheading:8819797-NADH, NADPH Oxidoreductases,
pubmed-meshheading:8819797-Reference Values
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pubmed:year |
1996
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pubmed:articleTitle |
Postcolumn co-immobilized leucine dehydrogenase-NADH oxidase reactor for the determination of branched-chain amino acids by high-performance liquid chromatography with chemiluminescence detection.
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pubmed:affiliation |
Department of Applied Chemistry and Biotechnology, Yamanashi University, Kofu, Japan.
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pubmed:publicationType |
Journal Article
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