Linked Life Data

8817093

Source:http://linkedlifedata.com/resource/pubmed/id/8817093

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-12-3
pubmed:abstractText
DNA ploidy and S-phase estimations are widely used as prognostic indicators in treatment decisions for breast cancer patients. For accurate calculation of the DNA index there is a need to identify the G0G1 diploid peak position. This study tested the reliability of peripheral blood mononuclear cells (PBMCs) as a diploid internal standard in the flow cytometric DNA analysis of 60 frozen malignant breast tumors. For each tumor, PBMCs were added to a duplicate sample prior to staining. There were 55 tumors with interpretable histograms; 18 were DNA diploid, 37 were DNA aneuploid, and all tumors had coefficients of variation < 5.0%. For each of the 55 tumors, whether diploid or aneuploid, the addition of PBMCs gave a unimodal peak at the diploid peak position. The results show that PBMCs are a reliable standard to identify the G0G1 diploid peak position.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0196-4763
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
161-5
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Peripheral blood mononuclear cells are a reliable internal standard for the flow cytometric DNA analysis of frozen malignant breast biopsies.
pubmed:affiliation
Department of Medicine, University of Sydney, New South Wales, Australia.
pubmed:publicationType
Journal Article