pubmed-article:8812855 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:8812855 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
pubmed-article:8812855 | lifeskim:mentions | umls-concept:C0024660 | lld:lifeskim |
pubmed-article:8812855 | lifeskim:mentions | umls-concept:C0035542 | lld:lifeskim |
pubmed-article:8812855 | lifeskim:mentions | umls-concept:C0071197 | lld:lifeskim |
pubmed-article:8812855 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
pubmed-article:8812855 | pubmed:issue | 2 | lld:pubmed |
pubmed-article:8812855 | pubmed:dateCreated | 1997-1-24 | lld:pubmed |
pubmed-article:8812855 | pubmed:abstractText | Affinity chromatography on immobilized ribonuclease (RNase) inhibitor was developed for purification of mammalian RNase. Human placental RNase inhibitor was conjugated to CNBr-activated Sepharose in the presence of dithiothreitol. About 80% of the immobilized RNase inhibitor was capable of binding bovine pancreatic RNase A. The bound RNase A was eluted with 3 M NaCl at pH 5.0. Two 25-kDa and 18-kDa RNases, which were obtained from human liver using a cellulose phosphate column, were bound to the immobilized RNase inhibitor and recovered in a pure and active form after treatment of the resin with p-hydroxymercuribenzoate. These enzymes were considered to be nonsecretory-type RNases with different sugar contents. | lld:pubmed |
pubmed-article:8812855 | pubmed:language | eng | lld:pubmed |
pubmed-article:8812855 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8812855 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:8812855 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8812855 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8812855 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8812855 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8812855 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8812855 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8812855 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8812855 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8812855 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8812855 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8812855 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:8812855 | pubmed:month | Mar | lld:pubmed |
pubmed-article:8812855 | pubmed:issn | 1046-5928 | lld:pubmed |
pubmed-article:8812855 | pubmed:author | pubmed-author:YasudaTT | lld:pubmed |
pubmed-article:8812855 | pubmed:author | pubmed-author:KishiKK | lld:pubmed |
pubmed-article:8812855 | pubmed:author | pubmed-author:TakeshitaHH | lld:pubmed |
pubmed-article:8812855 | pubmed:author | pubmed-author:NadanoDD | lld:pubmed |
pubmed-article:8812855 | pubmed:author | pubmed-author:SawazakiKK | lld:pubmed |
pubmed-article:8812855 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:8812855 | pubmed:volume | 7 | lld:pubmed |
pubmed-article:8812855 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:8812855 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:8812855 | pubmed:pagination | 167-72 | lld:pubmed |
pubmed-article:8812855 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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pubmed-article:8812855 | pubmed:year | 1996 | lld:pubmed |
pubmed-article:8812855 | pubmed:articleTitle | Purification of mammalian ribonuclease using immobilized human ribonuclease inhibitor. | lld:pubmed |
pubmed-article:8812855 | pubmed:affiliation | Department of Legal Medicine, Fukui Medical School, Japan. | lld:pubmed |
pubmed-article:8812855 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:8812855 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |