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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1997-1-24
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pubmed:abstractText |
Affinity chromatography on immobilized ribonuclease (RNase) inhibitor was developed for purification of mammalian RNase. Human placental RNase inhibitor was conjugated to CNBr-activated Sepharose in the presence of dithiothreitol. About 80% of the immobilized RNase inhibitor was capable of binding bovine pancreatic RNase A. The bound RNase A was eluted with 3 M NaCl at pH 5.0. Two 25-kDa and 18-kDa RNases, which were obtained from human liver using a cellulose phosphate column, were bound to the immobilized RNase inhibitor and recovered in a pure and active form after treatment of the resin with p-hydroxymercuribenzoate. These enzymes were considered to be nonsecretory-type RNases with different sugar contents.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1046-5928
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
167-72
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8812855-Amidohydrolases,
pubmed-meshheading:8812855-Animals,
pubmed-meshheading:8812855-Cation Exchange Resins,
pubmed-meshheading:8812855-Cattle,
pubmed-meshheading:8812855-Cellulose,
pubmed-meshheading:8812855-Chromatography, Affinity,
pubmed-meshheading:8812855-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8812855-Female,
pubmed-meshheading:8812855-Humans,
pubmed-meshheading:8812855-Hydrogen-Ion Concentration,
pubmed-meshheading:8812855-Isoenzymes,
pubmed-meshheading:8812855-Liver,
pubmed-meshheading:8812855-Molecular Weight,
pubmed-meshheading:8812855-Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase,
pubmed-meshheading:8812855-Placenta,
pubmed-meshheading:8812855-Placental Hormones,
pubmed-meshheading:8812855-RNA,
pubmed-meshheading:8812855-Ribonucleases,
pubmed-meshheading:8812855-Substrate Specificity
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pubmed:year |
1996
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pubmed:articleTitle |
Purification of mammalian ribonuclease using immobilized human ribonuclease inhibitor.
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pubmed:affiliation |
Department of Legal Medicine, Fukui Medical School, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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