Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1997-1-24
|
| pubmed:abstractText |
A one step rapid and simple purification procedure for trichosanthin, a type 1 ribosome inactivating protein, from root tubers of Trichosanthes kirilowii has been developed using cation-exchange perfusion chromatography. The identity of the protein has been confirmed by its size, immunoreactivity, and sequence information. Yields of 0.16% of electrophoretically pure trichosanthin from dried root tuber have been achieved with a single 10-min chromatographic step giving the ability to purify gram quantities of trichosanthin in 1 day.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
1046-5928
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
143-6
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8812847-Abortifacient Agents,
pubmed-meshheading:8812847-Blotting, Western,
pubmed-meshheading:8812847-Chromatography, Ion Exchange,
pubmed-meshheading:8812847-Drugs, Chinese Herbal,
pubmed-meshheading:8812847-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8812847-Plant Proteins,
pubmed-meshheading:8812847-Plants,
pubmed-meshheading:8812847-Trichosanthin
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
A simplified procedure for the purification of trichosanthin (a type 1 ribosome inactivating protein) from Trichosanthes kirilowii root tubers.
|
| pubmed:affiliation |
Department of Chemical Engineering and Materials Science, University of California at Davis 95616, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|