Linked Life Data

8812084

Source:http://linkedlifedata.com/resource/pubmed/id/8812084

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-10-23
pubmed:abstractText
A proportion of implanted cryopreserved allogeneic cardiac valves (ACV) fail due to tissue degeneration initiated by immunological reactions. This study was carried out in a rat model system [Brown Norway (BN; RT1(n)) to Lewis (RT1(l))] to determine the possibility of cryoimmunomodulation of ACV. The immunogenicity of fresh and cryopreserved (1 degrees, 5 degrees, 10 degrees, 30 degrees, and >100 degrees C/min) BN aortic valve conduits (AVC) was assessed using a mixed AVC cell/responder lymphocyte reaction. No significant differences (p > 0.05) in immunogenicity between fresh and cryopreserved (1 degrees C/min and 5 degrees C/min) AVC were observed between 120 and 168 h of co-culture. A significant reduction in immunogenicity was observed with AVC cryopreserved using cooling rates of 10 degrees, 30 degrees, and >100 degrees C/min. The viability of fresh and cryopreserved AVC was determined by a [3H]proline uptake assay. A decrease in viability was observed at cooling rates of more than 1 degrees C/min. The feasibility of cryoimmunomodulation of ACV with the maintenance of viability of a proportion of cells was demonstrated.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0011-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
41-53
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
The effect of cryopreservation on the immunogenicity of allogeneic cardiac valves.
pubmed:affiliation
Department of Microbiology, University of Leeds, Leeds, United Kingdom.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't