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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1996-11-22
|
| pubmed:abstractText |
Antibody to specific cell-wall carbohydrate of group A streptococci in human sera was determined by enzyme-linked immunosorbent assay (ELISA) with enzyme-treated whole cells and purified group A carbohydrate (ACHO) as antigens. The optimal concentration of enzyme-treated whole bacterial cells to coat wells was 2 x 10(8) cells/ml and for purified ACHO antigen the optimal concentration was 1 microgram/ml. Sera from patients with acute rheumatic fever and acute glomerulonephritis were screened for the presence of C-carbohydrate antibodies. Patients with acute post-streptococcal complications showed significantly higher titres of antibody when compared with normal healthy individuals. ELISA results were also compared with purified ACHO as an antigen; this showed a highly significant correlation (r = 0.73). Results showed that measurement of the anti-ACHO antibody by ELISA with enzyme-treated whole cells can be a useful, reliable and simple method for serological diagnosis of group A infection and sequelae.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0022-2615
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
45
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
214-8
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading | |
| pubmed:year |
1996
|
| pubmed:articleTitle |
Detection of antibody to C-carbohydrate of group A streptococci with enzyme-treated whole bacterial cells as antigen for ELISA.
|
| pubmed:affiliation |
WHO Reference Laboratory for Streptococcal Diseases, Department of Microbiology, Lady Hardinge Medical School, New Delhi, India.
|
| pubmed:publicationType |
Journal Article
|