Linked Life Data

8810280

Source:http://linkedlifedata.com/resource/pubmed/id/8810280

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
41
pubmed:dateCreated
1996-11-19
pubmed:abstractText
Autoflavinylation of 6-hydroxy-D-nicotine oxidase (6-HDNO) was successfully employed to modify the protein covalently with FAD derivatives. The model compounds N6-(2-aminoethyl)-FAD and N6-(6-carboxyhexyl)-FAD were spontaneously bound to a fusion protein consisting of the mitochondrial targeting sequence of Neurospora crassa F0-ATPase subunit 9 (Su9) attached to 6-HDNO. When translated in the rabbit reticulocyte lysate, Su9-6-HDNO was in the trypsin-sensitive apoenzyme form; when translated in the presence of flavins it adopted a trypsin-resistant conformation characteristic of the 6-HDNO holoenzyme. With flavin derivatives, Su9-6-HDNO exhibited approximately 50% of the 6-HDNO activity observed with FAD. The covalently modified Su9-6-HDNO was imported into Saccharomyces cerevisiae mitochondria with an efficiency equal to that of the apoenzyme. Apparently the increase in size and charge of the FAD moiety did not hamper translocation across the mitochondrial membranes. Yeast mutant ssc1-2 mitochondria deficient in mtHsp70 unfoldase activity imported the flavinylated Su9-6-HDNO protein. In mutant ssc1-3 mitochondria deficient in both mtHsp70 unfoldase and translocase activity Su9-6-HDNO was trapped as translocation intermediate; the Su9 presequence was passed to the matrix where it was proteolytically cleaved by the mitochondrial processing peptidase; (MPP); the translocation-arrested 6-HDNO moiety adopted a trypsin-sensitive conformation. Our results indicate that unfolding of the FAD-stabilized flavin-binding domain of 6-HDNO in passage through the mitochondrial general insertion pore does not require the activity of mtHsp70.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
271
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
25208-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8810280-Animals, pubmed-meshheading:8810280-DNA Primers, pubmed-meshheading:8810280-Flavin-Adenine Dinucleotide, pubmed-meshheading:8810280-HSP70 Heat-Shock Proteins, pubmed-meshheading:8810280-Mitochondria, pubmed-meshheading:8810280-Neurospora crassa, pubmed-meshheading:8810280-Oxidoreductases, pubmed-meshheading:8810280-Polymerase Chain Reaction, pubmed-meshheading:8810280-Protein Biosynthesis, pubmed-meshheading:8810280-Protein Folding, pubmed-meshheading:8810280-Protein Processing, Post-Translational, pubmed-meshheading:8810280-Proton-Translocating ATPases, pubmed-meshheading:8810280-Rabbits, pubmed-meshheading:8810280-Recombinant Fusion Proteins, pubmed-meshheading:8810280-Reticulocytes, pubmed-meshheading:8810280-Saccharomyces cerevisiae, pubmed-meshheading:8810280-Trypsin
pubmed:year
1996
pubmed:articleTitle
Covalent attachment of FAD derivatives to a fusion protein consisting of 6-hydroxy-D-nicotine oxidase and a mitochondrial presequence. Folding, enzyme activity, and import of the modified protein into yeast mitochondria.
pubmed:affiliation
Institut für Biochemie und Molekularbiologie, D-79104 Freiburg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't