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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5284
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pubmed:dateCreated |
1996-10-24
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pubmed:abstractText |
Identification and characterization of antigen-specific T lymphocytes during the course of an immune response is tedious and indirect. To address this problem, the peptide-major histocompatability complex (MHC) ligand for a given population of T cells was multimerized to make soluble peptide-MHC tetramers. Tetramers of human lymphocyte antigen A2 that were complexed with two different human immunodeficiency virus (HIV)-derived peptides or with a peptide derived from influenza A matrix protein bound to peptide-specific cytotoxic T cells in vitro and to T cells from the blood of HIV-infected individuals. In general, tetramer binding correlated well with cytotoxicity assays. This approach should be useful in the analysis of T cells specific for infectious agents, tumors, and autoantigens.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Coloring Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, gag,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A2 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Directed DNA Polymerase,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/influenza virus membrane protein...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
274
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
94-6
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pubmed:dateRevised |
2009-9-29
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pubmed:meshHeading |
pubmed-meshheading:8810254-Amino Acid Sequence,
pubmed-meshheading:8810254-Antigens, Viral,
pubmed-meshheading:8810254-Base Sequence,
pubmed-meshheading:8810254-CD8-Positive T-Lymphocytes,
pubmed-meshheading:8810254-Cell Line,
pubmed-meshheading:8810254-Coloring Agents,
pubmed-meshheading:8810254-Epitopes,
pubmed-meshheading:8810254-Flow Cytometry,
pubmed-meshheading:8810254-Gene Products, gag,
pubmed-meshheading:8810254-HIV Seropositivity,
pubmed-meshheading:8810254-HLA-A2 Antigen,
pubmed-meshheading:8810254-Humans,
pubmed-meshheading:8810254-Molecular Sequence Data,
pubmed-meshheading:8810254-Peptide Fragments,
pubmed-meshheading:8810254-Phenotype,
pubmed-meshheading:8810254-RNA-Directed DNA Polymerase,
pubmed-meshheading:8810254-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:8810254-Viral Matrix Proteins
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pubmed:year |
1996
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pubmed:articleTitle |
Phenotypic analysis of antigen-specific T lymphocytes.
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pubmed:affiliation |
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305-5428, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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