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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1997-3-31
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pubmed:abstractText |
The plasmid-encoded RepE protein is absolutely essential and rate-limiting for replication of the promiscuous plasmid pAM beta 1 originating from Enterococcus faecalis. We previously showed that the rep gene is transcribed from a promoter that is negatively regulated (approximately 10-fold reduction) by the CopF repressor. In this report, we show that this transcription is decreased a further approximately 10-times by a countertranscript-driven transcriptional attenuation system. Extensive mutagenesis revealed that this system operates by a mechanism similar to that previously described for the unrelated repC gene of plasmid pT181.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0950-382X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1099-112
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8809762-Bacterial Proteins,
pubmed-meshheading:8809762-DNA-Binding Proteins,
pubmed-meshheading:8809762-Enterococcus faecalis,
pubmed-meshheading:8809762-Escherichia coli Proteins,
pubmed-meshheading:8809762-Gene Dosage,
pubmed-meshheading:8809762-Gene Expression Regulation, Bacterial,
pubmed-meshheading:8809762-Mutagenesis,
pubmed-meshheading:8809762-Nucleic Acid Conformation,
pubmed-meshheading:8809762-Plasmids,
pubmed-meshheading:8809762-Promoter Regions, Genetic,
pubmed-meshheading:8809762-RNA, Bacterial,
pubmed-meshheading:8809762-Repressor Proteins,
pubmed-meshheading:8809762-Signal Transduction,
pubmed-meshheading:8809762-Transcription, Genetic
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pubmed:year |
1996
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pubmed:articleTitle |
Countertranscript-driven attenuation system of the pAM beta 1 repE gene.
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pubmed:affiliation |
Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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