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rdf:type |
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lifeskim:mentions |
umls-concept:C0010453,
umls-concept:C0011777,
umls-concept:C0032016,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0035696,
umls-concept:C0037663,
umls-concept:C0037668,
umls-concept:C0229535,
umls-concept:C0441889,
umls-concept:C0597357,
umls-concept:C0815016,
umls-concept:C2751021
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pubmed:issue |
6
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pubmed:dateCreated |
1996-11-21
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pubmed:abstractText |
To examine the effects of glucocorticoid (GC) on growth hormone (GH)-releasing hormone (GRH) receptor gene expression, a highly-sensitive and quantitative reverse-transcribed polymerase chain reaction (RT-PCR) method was used in this study. Rat anterior pituitary cells were isolated and cultured for 4 days. The cultured cells were treated with dexamethasone for 2, 6, and 24 h. GRH receptor mRNA levels were determined by competitive RT-PCR using a recombinant RNA as the competitor. Dexamethasone significantly increased GRH receptor mRNA levels at 5 nM after 6- and 24 h-incubations, and the maximal effect was found at 25 nM. The GC receptor-specific antagonist, RU 38486 completely eliminated the dexamethasone-induced enhancement of GRH receptor mRNA levels. Dexamethasone did not alter the mRNA levels of beta-actin and prolactin at 5 nM for 24 h, whereas GH mRNA levels were significantly increased by the same treatment. The GH response to GRH was significantly enhanced by the 24-h incubation with 5 nM dexamethasone. These findings suggest that GC stimulates GRH receptor gene expression through the ligand-activated GC receptors in the rat somatotrophs. The direct effects of GC on the GRH receptor gene could explain the enhancement of GRH-induced GH secretion.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Hormone-Releasing Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Mifepristone,
http://linkedlifedata.com/resource/pubmed/chemical/Prolactin,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Directed DNA Polymerase,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Neuropeptide,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Pituitary...,
http://linkedlifedata.com/resource/pubmed/chemical/somatotropin releasing hormone...
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0953-8194
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
475-80
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8809678-Actins,
pubmed-meshheading:8809678-Animals,
pubmed-meshheading:8809678-Binding, Competitive,
pubmed-meshheading:8809678-Cells, Cultured,
pubmed-meshheading:8809678-DNA Primers,
pubmed-meshheading:8809678-Dexamethasone,
pubmed-meshheading:8809678-Dose-Response Relationship, Drug,
pubmed-meshheading:8809678-Gene Expression,
pubmed-meshheading:8809678-Growth Hormone,
pubmed-meshheading:8809678-Growth Hormone-Releasing Hormone,
pubmed-meshheading:8809678-Kinetics,
pubmed-meshheading:8809678-Male,
pubmed-meshheading:8809678-Mifepristone,
pubmed-meshheading:8809678-Pituitary Gland, Anterior,
pubmed-meshheading:8809678-Polymerase Chain Reaction,
pubmed-meshheading:8809678-Prolactin,
pubmed-meshheading:8809678-RNA, Messenger,
pubmed-meshheading:8809678-RNA-Directed DNA Polymerase,
pubmed-meshheading:8809678-Rats,
pubmed-meshheading:8809678-Rats, Wistar,
pubmed-meshheading:8809678-Receptors, Neuropeptide,
pubmed-meshheading:8809678-Receptors, Pituitary Hormone-Regulating Hormone
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pubmed:year |
1996
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pubmed:articleTitle |
Dexamethasone increases growth hormone (GH)-releasing hormone (GRH) receptor mRNA levels in cultured rat anterior pituitary cells.
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pubmed:affiliation |
First Department of Internal Medicine, Kagawa Medical School, Japan.
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pubmed:publicationType |
Journal Article
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