Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1996-11-22
pubmed:abstractText
Quinolone-resistant clinical Escherichia coli isolates were examined for mutations in the marRAB operon of the multiple antibiotic resistance (mar) locus. Among 23 strains evaluated, 8 were chosen for further study: 3 that showed relatively high levels of uninduced, i.e., constitutive, expression of the operon and 5 with variable responses to induction by salicylate or tetracyclines. The marR genes, specifying the repressor of the operon, cloned from the three strains constitutively expressing the operon did not reduce the level of expression of beta-galactosidase from a marO::lacZ transcriptional fusion and were therefore mutant; however, marR genes cloned from the five other clinical strains repressed LacZ expression and were wild type. All three mutant marR genes contained more than one mutation: a deletion and a point mutation. Inactivation of the mar locus in the three known marR mutant strains with a kanamycin resistance cassette introduced by homologous recombination reduced resistance to quinolones and multiple antibiotics. These findings indicate that mar operon mutations exist in quinolone-resistant clinical E. coli isolates and contribute to quinolone and multidrug resistance.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-1509257, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-1592705, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-1715857, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-2127399, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-2548993, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-2655532, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-2679373, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-2829712, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-2848006, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-3000292, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-3605586, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-6307967, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-6348022, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-7504664, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-7768850, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-7777530, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-8031031, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-8058823, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-8139646, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-8282690, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-8335992, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-8354301, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-8383113, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807064-8723480
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0066-4804
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1695-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Identification for mar mutants among quinolone-resistant clinical isolates of Escherichia coli.
pubmed:affiliation
Center for Adaptation Genetics and Drug Resistance, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.