Linked Life Data

8806616

Source:http://linkedlifedata.com/resource/pubmed/id/8806616

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-10-22
pubmed:abstractText
In a search for new inhibitors of NF-kappa B mediated signal transduction in COS-7 cells using the Secreted Alkaline Phosphatase as reporter gene, panepoxydone has been isolated from fermentations of the basidiomycete Lentinus crinitus. Panepoxydone inhibits the NF-kappa B activated expression of the SEAP with an IC50 of 1.5-2 micrograms/ml (7.15-9.52 microM). No inhibition of AP-1 mediated expression of the reporter gene could be observed at a concentration up to 5 micrograms/ml panepoxydone. Panepoxydone strongly reduced the TPA, TNF-alpha and ocadaic acid mediated binding of NF-kappa B to the high affinity consensus sequence in COS-7 and HeLa S3 cells as confirmed by EMSA's. Panepoxydone inhibits the I kappa B phosphorylation and therefore sequesteres the NF-kappa B complex in an inactive form.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
226
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
214-21
pubmed:dateRevised
2006-5-1
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Inhibition of NF-kappa B activation by panepoxydone.
pubmed:affiliation
Lehrbereich Biotechnologie der Universität, Kaiserslautern, Germany. erkel@rhrk.uni-kl.de
pubmed:publicationType
Journal Article