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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1996-12-5
|
| pubmed:abstractText |
A detailed mapping of the gamma-aminobutyric acid (GABA)A receptor subunits (alpha 1, alpha 2, alpha 3, alpha 5 and beta 2 + 3) in the infralimbic/ventral prelimbic region (IL/vPL) of the rat frontal cortex was carried out using subunit-specific antibodies. The alpha 1 and beta 2 + 3 subunit antibodies immunostained all layers of the IL/vPL region. Layers II and III displayed immunostaining of cell bodies whereas I, V and VI showed predominantly neuropil staining. The size of the alpha 1-positive cell bodies corresponded to that of small interneurons (range, 20-55 microns2; mean +/- SEM, 37 +/- 5.5 microns2) as well as pyramidal cells or large interneurons (range, 87-135 microns2; mean +/- SEM, 103.4 +/- 9.7 microns2). However, beta 2 + 3 antibody immunostained only small cell bodies. Immunoreactivity for alpha 2 was restricted to layers I and II, whereas alpha 3 and alpha 5 subunit expression was seen only in layer VI. The antibody to the alpha 2 subunit immunostained small cell bodies (range, 29-63 microns2; mean +/- SEM, 32 +/- 4.5 microns2) in layer II, resembling interneurons. Conversely, both alpha 3 and alpha 5 antibodies immunostained large cell bodies (range, 94-151 microns2; mean +/- SEM, 115.7 +/- 13.4 microns2), consistent with pyramidal cell labelling in layer VI.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0304-3940
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
7
|
| pubmed:volume |
210
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
213-7
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8805133-Animals,
pubmed-meshheading:8805133-Antibodies, Monoclonal,
pubmed-meshheading:8805133-Immunohistochemistry,
pubmed-meshheading:8805133-Interneurons,
pubmed-meshheading:8805133-Prefrontal Cortex,
pubmed-meshheading:8805133-Pyramidal Cells,
pubmed-meshheading:8805133-Rats,
pubmed-meshheading:8805133-Receptors, GABA-A
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Differential distribution of gamma-aminobutyric acidA receptor subunit (alpha 1, alpha 2, alpha 3, alpha 5 and beta 2 + 3) immunoreactivity in the medial prefrontal cortex of the rat.
|
| pubmed:affiliation |
Pharmacia and Upjohn, Inc., Kalamazoo, MI 49001, USA.
|
| pubmed:publicationType |
Journal Article
|