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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1996-10-16
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pubmed:abstractText |
Proteolytic and nonproteolytic methods were used to investigate the mechanism(s) by which human fibroblast progelatinase A and fibroblast-type procollagenase can be activated. Both collagenase and matrilysin were able to activate progelatinase A, resulting in an amino terminus in gelatinase A of Tyr81. The cleavage occurred distal to Cys73 within the sequence of PRCGNPDVAN80-Y81NFFPRKP. While several nonproteolytic reagents were tested, only the heavy metal Hg(II) and p-chloromercuribenzoate (PCMB) were able to induce activation of progelatinase A and resulted in the conversion of the latent 72-kDa gelatinase A to an active form of about 64.5 kDa. Matrilysin was also able to activate procollagenase and resulted in an amino terminus in collagenase of Phe81. These results suggest that fibroblast-type collagenase and matrilysin may be physiologically relevant activators of progelatinase A; the maintenance of latency and the process of activation for progelatinase A may occur through the cysteine-switch mechanism, and the proteolytic activation of procollagenase by matrilysin resulted in the same amino terminus as produced by stromelysin-1.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0277-8033
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
243-53
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8804571-Amino Acid Sequence,
pubmed-meshheading:8804571-Animals,
pubmed-meshheading:8804571-Collagenases,
pubmed-meshheading:8804571-Dose-Response Relationship, Drug,
pubmed-meshheading:8804571-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8804571-Enzyme Activation,
pubmed-meshheading:8804571-Enzyme Precursors,
pubmed-meshheading:8804571-Fibroblasts,
pubmed-meshheading:8804571-Gelatinases,
pubmed-meshheading:8804571-Gingiva,
pubmed-meshheading:8804571-Humans,
pubmed-meshheading:8804571-Matrix Metalloproteinase 3,
pubmed-meshheading:8804571-Matrix Metalloproteinase 7,
pubmed-meshheading:8804571-Metalloendopeptidases,
pubmed-meshheading:8804571-Molecular Sequence Data,
pubmed-meshheading:8804571-Rats,
pubmed-meshheading:8804571-Sequence Analysis,
pubmed-meshheading:8804571-Silver Staining,
pubmed-meshheading:8804571-Time Factors
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pubmed:year |
1996
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pubmed:articleTitle |
Activation of human progelatinase A by collagenase and matrilysin: activation of procollagenase by matrilysin.
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pubmed:affiliation |
Department of Chemistry, Florida State University, Tallahassee 32306-3006, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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