|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
17
|
|
pubmed:dateCreated |
1996-11-7
|
|
pubmed:abstractText |
The paired box transcription factor Pax-5 (B-cell-specific activator protein) is a key regulator of lineage-specific gene expression and differentiation in B-lymphocytes. We show that Pax-5 functions as a cell type-specific docking protein that facilitates binding of the early B-cell-specific mb-1 promoter by proteins of the Ets proto-oncogene family. Transcriptional activity of the mb-1 promoter in pre-B-cells is critically dependent on binding sites for Pax-5:Ets complexes. Ternary complex assembly requires only the Pax-5 paired box and ETS DNA-binding domains. Mutation of a single base pair in the ternary complex binding site allows for independent binding by Ets proteins but, conversely, inhibits the binding of Pax-5 by itself. Strikingly, the mutation reverses the pattern of complex assembly: Ets proteins recruit Pax-5 to bind the mutated sequence. Recruitment of Net and Elk-1, but not SAP1a, by Pax-5 defines a functional difference between closely related Ets proteins. Replacement of a valine (V68) in the ETS domain of SAP1a by aspartic acid (as found in c-Ets-1, Elk-1, and Net) enhanced ternary complex formation by more than 60-fold. Together, these observations define novel transcription factor interactions that regulate gene expression in B cells.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD79,
http://linkedlifedata.com/resource/pubmed/chemical/B-Cell-Specific Activator Protein,
http://linkedlifedata.com/resource/pubmed/chemical/CD79A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ELK1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Elk3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PAX5 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-ets,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, B-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Sap1 protein, S pombe,
http://linkedlifedata.com/resource/pubmed/chemical/Schizosaccharomyces pombe Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/ets-Domain Protein Elk-1
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0890-9369
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
10
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2198-211
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:8804314-Amino Acid Sequence,
pubmed-meshheading:8804314-Antigens, CD,
pubmed-meshheading:8804314-Antigens, CD79,
pubmed-meshheading:8804314-B-Cell-Specific Activator Protein,
pubmed-meshheading:8804314-B-Lymphocytes,
pubmed-meshheading:8804314-Base Sequence,
pubmed-meshheading:8804314-Binding Sites,
pubmed-meshheading:8804314-DNA-Binding Proteins,
pubmed-meshheading:8804314-Gene Expression Regulation,
pubmed-meshheading:8804314-Humans,
pubmed-meshheading:8804314-Molecular Sequence Data,
pubmed-meshheading:8804314-Mutagenesis,
pubmed-meshheading:8804314-Nuclear Proteins,
pubmed-meshheading:8804314-Oncogene Proteins,
pubmed-meshheading:8804314-Plasmids,
pubmed-meshheading:8804314-Point Mutation,
pubmed-meshheading:8804314-Promoter Regions, Genetic,
pubmed-meshheading:8804314-Protein Binding,
pubmed-meshheading:8804314-Protein Biosynthesis,
pubmed-meshheading:8804314-Proto-Oncogene Proteins,
pubmed-meshheading:8804314-Proto-Oncogene Proteins c-ets,
pubmed-meshheading:8804314-Receptors, Antigen, B-Cell,
pubmed-meshheading:8804314-Recombination, Genetic,
pubmed-meshheading:8804314-Schizosaccharomyces pombe Proteins,
pubmed-meshheading:8804314-Transcription, Genetic,
pubmed-meshheading:8804314-Transcription Factors,
pubmed-meshheading:8804314-Transfection,
pubmed-meshheading:8804314-ets-Domain Protein Elk-1
|
|
pubmed:year |
1996
|
|
pubmed:articleTitle |
Pax-5 (BSAP) recruits Ets proto-oncogene family proteins to form functional ternary complexes on a B-cell-specific promoter.
|
|
pubmed:affiliation |
Division of Basic Immunology, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
